Effects of Shugan Lipi decoction on intestinal flora in non-alcoholic steatohepatitis rats
10.13699/j.cnki.1001-6821.2024.17.016
- VernacularTitle:疏肝理脾汤对非酒精性脂肪性肝炎大鼠肠道菌群的影响
- Author:
Yuan-Yuan SHI
1
;
Ya WANG
;
Dan GUO
;
Hang-Yu ZHONG
;
Yun-Jie ZHENG
;
Tao ZHANG
Author Information
1. 湖南中医药大学第一附属医院肝病科,湖南长沙 410007
- Keywords:
Shugan Lipi decoction;
nonalcoholic steatohepatitis;
Toll-like receptor 4;
T cell immunoglobulin domain and mucin domain-3;
intestinal flora;
inflammation
- From:
The Chinese Journal of Clinical Pharmacology
2024;40(17):2533-2537
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effect of Shugan Lipi decoction on inflammation and intestinal flora,Toll like receptor 4(TLR4),T cell immunoglobulin domain and mucin domain-3(Tim-3)in non-alcoholic steatohepatitis(NASH)rats.Methods The NASH model was established by feeding methionine and choline deficient diet for 4 weeks.SD rats were randomly divided into blank group(intragastric administration with 0.9％NaCl),model group(NASH model,intragastric administration with 0.9％NaCl),and experimental group(NASH model,intragastric administration with 6.18g·kg-1 Shugan Lipi decoction).Illumina sequencing by synthesis method was used to detect the 16S rRNA sequence of rat Intestinal microbiota.Western blot method was used to detect the expression levels of Tim-3 and TLR4 proteins.Enzyme-linked immunosorbent assay was used to detect tumor necrosis factor-α(TNF-α),interleukin(IL)-6 and IL-10 levels in each group of rats.Results After 4 weeks of medication,the relative abundance of Bacteroidetes in the blank,model and experimental groups were(47.96±10.52)％,(42.90±15.01)％and(57.15±10.99)％;the relative abundance of Firmicutes were(49.27±9.99)％,(53.06±11.47)％and(39.99±11.88)％;the relative expression levels of Tim-3 protein were 1.03±0.07,0.24±0.06 and 1.57±0.11;the relative expression levels of TLR4 protein were 1.04±0.11,3.23±0.33 and 0.94±0.27;the levels of TNF-α were(403.03±25.25),(576.87±60.29)and(385.16±37.67)pg·mL-1;the levels of IL-6 were(125.35±14.07),(189.75±34.30)and(113.71±18.35)pg·mL-1;the levels of IL-10 were(123.20±15.96),(66.71±11.94)and(119.54±10.57)pg·mL-1,respectively.The above indexes in the experimental group showed statistically significant differences compared with the model group(P＜0.01,P＜0.05).Conclusion Shugan Lipi decoction may regulate inflammatory cytokines by affecting intestinal flora and TLR4,Tim-3 protein expression,affect liver inflammatory response,and improve NASH.
