Hypericin inhibits the expression of NLRP3 in microglia of Parkinson's disease mice and alleviates the damage of DA-ergic neurons
10.13699/j.cnki.1001-6821.2024.17.014
- VernacularTitle:金丝桃苷抑制帕金森病小鼠小胶质细胞NLRP3表达缓解DA能神经元损伤的研究
- Author:
Li-Shan FAN
1
;
Jia ZHANG
;
Si-Xiang NIU
;
Qi XIAO
;
Hui-Jie FAN
;
Lei XU
;
Li-Xia YANG
;
Lu JIA
;
Shao-Chen QIN
;
Bao-Guo XIAO
;
Cun-Gen MA
;
Zhi CHAI
Author Information
1. 山西中医药大学多发性硬化益气活血重点研究室/神经生物学研究中心,山西晋中 030619
- Keywords:
hyperoside;
Parkinson's disease;
Nod-like receptor heat protein domain protein 3
- From:
The Chinese Journal of Clinical Pharmacology
2024;40(17):2523-2527
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the intervention effect of hypericin(HYP)on 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine(MPTP)-induced Parkinson's disease(PD)mice model and its mechanism.Methods Thirty C57BL/6 mice were randomly divided into normal,model and experimental groups with 10 mice per group.PD mouse model was established after 7 days of intraperitoneal injection of MPTP,and drug intervention was carried out from the first day of modeling.Normal group and model group were intraperitoneally injected with 500 μL·kg·d-1 0.9％NaCl.The experimental group was intraperitoneally injected with 25 mg·kg·d-1 HYP.The three groups of rats were given the drug once each time for 14 days.The expression levels of tyrosine hydroxylase(TH),Nod-like receptor thermal protein domain protein 3(NLRP3)and ionized calcium binding adapter molecule 1(Iba1)in the striatum of nigra were detected by Western blot.Results The climbing time of normal,model and experimental groups was(5.35±0.43),(9.71±1.19)and(8.07±0.34)s;suspension scores were(2.92±0.15),(1.38±0.28)and(1.96±0.28)points;the relative expression levels of TH protein were 1.04±0.06,0.51±0.09 and 0.75±0.07;the relative expression levels of NLRP3 protein were 0.51±0.03,1.00±0.04 and 0.77±0.06;the relative expression levels of Iba1 protein were 0.68±0.10,1.30±0.28 and 0.89±0.05,respectively.The above indexes in the model group were statistically significant compared with the experimental group and the normal group(all P＜0.01).Conclusion HYP plays a therapeutic role in PD by inhibiting the expression of NLRP3 inflammasome in PD mice.
