Mechanism analysis of E7766 in the treatment of bladder cancer based on network pharmacology and bioinformatics
10.13699/j.cnki.1001-6821.2024.16.023
- VernacularTitle:基于网络药理学及生物信息学研究E7766治疗膀胱癌的作用分析
- Author:
Tian-Tian QI
1
,
2
,
3
;
Yu-Hang ZHANG
;
Yi-Min CUI
Author Information
1. 徐州医科大学药学院,江苏徐州 221004
2. 北京大学第一医院,临床药理研究所,北京 100034
3. 北京大学第一医院,药学部,北京 100034
- Keywords:
E7766;
bladder cancer;
network pharmacology;
bioinformatics analysis
- From:
The Chinese Journal of Clinical Pharmacology
2024;40(16):2409-2412
- CountryChina
- Language:Chinese
-
Abstract:
Objective This article aims to explore the target and mechanism of E7766 in the treatment of bladder cancer through network pharmacology and online biological analysis.Methods Relevant targets of E7766 and bladder cancer were obtained with the help of multiple drug and disease databases.The core target of E7766 in the treatment of bladder cancer was determined by using Cytoscape software.Perform gene ontology(GO)analysis and Kyoto encyclopedia of genes and genomes(KEGG)enrichment pathway analysis on the selected targets through the David database.The Gene Expression Profiling Interactive Analysis(GEPIA)database was used to analyze the expression of core targets in bladder cancer and their relationship with patient prognosis.Perform immune infiltration analysis on core target genes using the Tumor Immune Estimation Resource(TIMER)database.Results Obtained drug disease core gene targets:caspase-3(CASP3),matrix metalloproteinase-9(MMP9),mammalian target of rapamycin(mTOR),prostaglandin endoperoxide synthase 2(PTGS2)and mitogen activated protein kinase 1(MAPK1).KEGG enrichment analysis showed that multiple pathways such as apoptosis pathway and tumor necrosis factor(TNF)signaling pathway had therapeutic effects on bladder cancer.GEPIA database analysis showed that MMP9 was highly expressed in bladder cancer tissues,while PTGS2 was low(P＜0.05);the expression level of MAPK1 is negatively correlated with prognosis,and the lower the expression of MAPK,the better the prognosis of patients(P＜0.05).The TIMER tumor immune database has found that core targets are closely related to the infiltration levels of various immune cells,playing an important role in the infiltration of dendritic cells and CD8+T cells.Conclusion E7766 influences biological processes such as apoptosis and inflammation of bladder cancer cells through multiple targets such as CASP3,MMP9,mTOR,PTGS2,MAPK1 and multiple pathways such as apoptosis and TNF signaling pathway.
