Astragaloside Ⅳ ameliorates irradiation-induced cardiomyocyte edema by modulating fluid metabolism
10.13699/j.cnki.1001-6821.2024.13.006
- VernacularTitle:黄芪甲苷通过调节水液代谢改善辐射诱导心肌细胞水肿的研究
- Author:
Shang-Zu ZHANG
1
;
Li-Ying ZHANG
;
Qi-Yang LI
;
Yan CHEN
;
Yang-Yang LI
;
Yong-Qi LIU
Author Information
1. 甘肃中医药大学甘肃省高校重大疾病分子医学与中医药防治研究重点实验室敦煌医学与转化教育部重点实验室,甘肃兰州 730000
- Keywords:
Astragaloside Ⅳ;
radiation;
myocardial edema;
hypoxia inducible factor-1α;
aquaporin 4
- From:
The Chinese Journal of Clinical Pharmacology
2024;40(13):1874-1877
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effectiveness of astragalosideⅣ(AS-Ⅳ)in ameliorating radiation-induced cardiomyocyte edema and its molecular mechanism.Methods The AC 16 cells were divided into blank group,model group,AS-Ⅳ pre-irradiation administration group(Q group),post-irradiation administration group(H group)and pre-post-irradiation administration group(Q+H group).H group was incubated with 40 mol·L-1 AS-Ⅳ drug-containing medium,and the other groups were incubated with normal medium for 24 h.After 24 h of incubation,6 Gy X-ray irradiation was given to establish the cardiomyocyte edema model in all groups except the blank group,and group Q was replaced with normal medium,and group H was replaced with 40 mol·L-1 AS-Ⅳ drug-containing medium for 24 h of incubation.Calcein AM staining was used to observe the edema of AC 16 cells,Western blot method was used to detect the expression of key proteins of aqueous fluid metabolism,and Annexin-V/PI flow apoptosis assay was used to detect apoptosis of AC 16 cells.Results The area of cellular edema in blank,model,Q,H,and Q+H groups were 690.77±199.55,1 184.47±307.36,713.65±152.48,809.72±262.85 and 897.61±213.66;the relative expression levels of hypoxia inducible factor(HIF)-1α protein were 0.94±0.02,1.35±0.03,0.91±0.03,0.69±0.02 and 0.86±0.03;the relative expression levels of aquaporin 4(AQP4)protein were 0.66±0.03,1.07±0.04,0.67±0.02,0.56±0.03 and 0.56±0.02;the apoptosis rates were(3.90±0.76)％,(16.58±0.63)％,(12.91±0.51)％,(14.05±0.22)％and(12.13±0.38)％,respectively.Statistically significant differences were found between the above indicators in the blank,Q,H and Q+H groups when compared to the model group(all P＜0.05).Conclusion AS-Ⅳ can regulate water-liquid metabolism,ameliorate radiation-induced cardiomyocyte edema,and attenuate apoptosis by inhibiting the abnormal activation of the HIF-1 α/AQP4 axis in irradiated AC 16 cells.
