Effect of palmatine inhibiting migration,invasion and epithelial mesenchymal transformation in human oral cancer KB cells
10.13699/j.cnki.1001-6821.2024.12.011
- VernacularTitle:巴马汀抑制人口腔癌KB细胞迁移、侵袭及上皮间充质转化的研究
- Author:
Xue-Yun CHENG
1
;
Guang-Yao HU
;
Hong-Li LIU
;
Chen-Guang LIU
;
Yuan-Li DING
;
Hui-Ning YANG
;
Yi-An ZHAO
;
Zhi-Guang SUN
Author Information
1. 吉林省人民医院口腔科,吉林长春 130021
- Keywords:
palmatine;
oral cancer;
epithelial mesenchymal transformation;
migration;
invasion
- From:
The Chinese Journal of Clinical Pharmacology
2024;40(12):1749-1753
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of palmatine on migration,invasion and epithelial mesenchymal transformation(EMT)in human oral cancer KB cells.Methods KB cells were divided into control group and palmatine-L,-M,-H groups,cultured with 0,4,8 and 16 μmol·L-1 palmatine.After incubation for 48 h,scratch assay was used to detect migration;Traswell assay was used to detect invasion;matrix metalloproteinase 2(MMP-2),MMP-9 and fibronectin(FN)contents were detected by enzyme-linked immunosorbent assay;the expression of Vimentin,N-cadherin and E-cadherin mRNA were detected by real-time quantitative polymerase chain reaction;the expression of Vimentin,N-cadherin,E-cadherin,Wnt3 and β-catenin protein were detected by Western blot.Results Cell mobility in control group and palmatine-L,-M,-H groups were(69.27±8.62)％,(52.94±4.49)％,(45.22±5.05)％and(37.63±4.88)％;the number of transmembrane cells were 197.33±20.26,125.33±12.01,97.00±9.17 and 62.67±7.51;the content of MMP-2 were(2.93±0.21),(1.49±0.13),(1.16±0.15)and(0.95±0.09)ng·mL-1;the content of MMP-9 were(3.51±0.36),(2.37±0.23),(2.06±0.35)and(1.72±0.16)ng·mL-1;the content of FN were(41.28±4.02),(24.03±3.17),(20.67±2.63)and(13.82±2.19)ng·mL-1;the above indexes in palmatine-L,-M,-H groups were compared with the control group,and the differences were statistically significant(P＜0.05,P＜0.01).The mRNA and protein expressions of Vimentin,N-cadherin and E-cadherin,and the expressions of Wnt3 and β-catenin protein in palmatine-L,-M,-H groups were statistically significant compared with those in control group(P＜0.05,P＜0.01).Conclusion Palmatine can inhibit the migration,invasion and EMT of human oral cancer KB cells,and its mechanism is related to the regulation of Wnt/β-catenin signaling pathway.
