Effects of hydroxysafflor yellow A on autophagy in bEnd.3 cells after oxygen-glucose deprivation
10.13699/j.cnki.1001-6821.2024.12.008
- VernacularTitle:羟基红花黄色素A对糖氧剥夺后bEnd.3细胞自噬作用的影响
- Author:
Yao-Yao DAI
1
;
Meng-Qi SHU
;
Ru-Heng WEI
;
Zhu-Yue MIAO
;
Zhi-Bin DING
;
Dong MA
;
Jian-Jun HUANG
;
Li-Juan SONG
;
Cun-Gen MA
Author Information
1. 山西中医药大学神经生物学研究中心,国家中医药管理局多发性硬化益气活血重点研究室,山西晋中 030619
- Keywords:
hydroxysafflor yellow A;
oxygen-glucose deprivation;
autophagy;
bEnd.3 cells;
sirtuin-1/forkhead box protein O3 a pathway
- From:
The Chinese Journal of Clinical Pharmacology
2024;40(12):1734-1738
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effect and mechanism of hydroxysafflor yellow A(HSYA)on autophagy in bEnd.3 cells after oxygen-glucose deprivation(OGD).Methods The bEnd.3 cells were divided into normal group(conventional culture),model group(OGD model),HSYA group(OGD model+75 μmol·L-1 HSYA),3-methyladenine(3MA)group(5 mmol·L-1 3MA+OGD model)and 3 MA+HSYA group(5 mmol·L-1 3 MA+OGD model+75 μmol·L-1 HSYA).The level of apoptosis was determined by TUNEL fluorescence staining;Western blot was used to detect the expression of autophagy,blood brain barrier(BBB)related proteins;real time fluorescence quantitative polymerase chain reaction method for determining the expression of sirtuin-1(SIRT1)and forkhead box protein O3a(FOXO3A)mRNA.Results In the normal group,model group,HSYA group,3MA group and 3MA+HSYA group,the positive cells selected for TUNEL staining were 5.00±1.00,28.00±2.00,21.00±3.00,35.33±2.51 and 29.67±2.52;the expression levels of microtubule-associated protein 1 light chain 3-Ⅱ/-Ⅰ(LC3-Ⅱ/-Ⅰ)were 0.90±0.20,1.34±0.10,1.95±0.14,0.76±0.15 and 1.14±0.09;sequestosome 1(P62)were 0.99±0.02,0.60±0.02,0.38±0.01,0.67±0.04 and 0.54±0.01;occludin were 1.39±0.17,0.62±0.15,1.00±0.09,0.40±0.13 and 0.80±0.15;zonula occludens-1(ZO-1)were 1.63±0.20,0.64±0.06,0.98±0.14,0.37±0.14 and 0.87±0.04;SIRT1 mRNA were 1.00±0.00,0.75±0.07,1.69±0.09,0.31±0.02 and 0.56±0.01;FOXO3A mRNA were 1.00±0.00,0.80±0.05,1.47±0.09,0.40±0.01 and 0.62±0.09,respectively.Significant differences were found between model group and normal group,HSYA group and model group,3MA+HSYA group and 3MA group(P＜0.05,P＜0.01,P＜0.001).Conclusion HSYA may enhance autophagy levels in bEnd.3 cells after OGD through the SIRT1/FOXO3A pathway,inhibit cell apoptosis and alleviate BBB damage.
