Simultaneous determination of 6 aconitum-type alkaloids in Xiaozhong ointment by ultra-high performance liquid chromatography triple quadrupole mass spectrometry
10.13699/j.cnki.1001-6821.2024.09.021
- VernacularTitle:超高效液相色谱-三重四极杆质谱法同时测定消肿膏中6种乌头型生物碱含量
- Author:
Tian-Li XIA
1
;
De-Ping ZHAO
;
Ao XUE
;
Mei-Ling JIN
;
Zi-Yue ZHU
;
Qing LI
;
Ning ZHANG
;
Xia LEI
Author Information
1. 佳木斯大学药学院,黑龙江佳木斯 154007
- Keywords:
Xiaozhong ointment;
aconitine alkaloid;
ultra-high performance liquid chromatography triple quadrupole mass spectrometry;
determination
- From:
The Chinese Journal of Clinical Pharmacology
2024;40(9):1341-1344
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a method for the simultaneous determination of aconitine,neoaconitine,hypaconitine,benzoyl aconitine,benzoyl mesaconine and benzoylhypacoitine in Xiaozhong ointment by UPLC-TQD-MS.Methods ACQUITY UPLC BEH C18 column(50 mm ×2.1 mm,1.7 μm),mobile phase 0.1％formic acid water(A)-acetonitrile(B),gradient elution,column temperature 40 ℃,flow rate 0.3 mL·min-1,injection volume 5 μL;electrospray ionization source(ESI+)and multiple reaction monitoring(MRM)were used for mass spectrometry analysis.Results The concentration of aconitine,new aconitine,hypaconitine,benzoyl aconitine,benzoyl new aconitine and benzoyl hypaconitine were 1.0-100.0 ng·mL-1,respectively,the average recovery were 98.62％-101.24％.The mass fractions of the six components were 0.18,0.33,0.38,0.43,0.28,0.06μg·g-1.Conclusion The method can be used to determine the content of 6 aconitine-type alkaloids in Xiaozhong ointment,and provide reference for the quality evaluation and clinical safe use of Xiaozhong ointment.
