Study on the effect of synaptic nuclear protein γ on migration and invasion of oral squamous cell carcinoma cells
10.13699/j.cnki.1001-6821.2024.09.006
- VernacularTitle:突触核蛋白γ对口腔鳞癌细胞迁移和侵袭作用的研究
- Author:
Zuo-Dong REN
1
;
Zhao-Wei ZHUANG
;
Juan ZHAO
;
Wu-Mei YUAN
;
Yan ZENG
Author Information
1. 石河子大学医学院新疆地方与民族高发病重点实验室,新疆维吾尔自治区石河子 832000
- Keywords:
synaptic nuclear protein γ;
oral squamous cell carcinoma;
lentiviral vector;
migration;
invasion
- From:
The Chinese Journal of Clinical Pharmacology
2024;40(9):1267-1271
- CountryChina
- Language:Chinese
-
Abstract:
Objective Lentivirus-mediated interference with synaptic nuclear protein γ(SNCG)in human oral squamous cell carcinoma was established to study the role of SNCG in the migration and invasion of oral squamous cell carcinoma.Methods Oral cancer CAL27 cells were infected with LV-shNC and LV-shSNCG constructed by lentivirus vector,respective,and then selected with puromycin to obtain cell lines stably interfering with SNCG,which were named NC group and experimental group,respectively.Detect the expression of SNCG through real-time quantitative polymerase chain reaction(qRT-PCR)and Western blot experiments;Transwell and scratch experiments were used to detect changes in migration and invasion ability.Results Compared with the NC group,the experimental group showed an 80％reduction in SNCG mRNA expression(P＜0.01).The relative expression level of SNCG protein was also decreased in the experimental group compared to the NC group(P＜0.01).In the NC group and the experimental group,the migration area percentages at 36 hours were 0.54±0.06 and 0.40±0.02,respectively;and at 48 hours were 0.83±0.01 and 0.47±0.05,respectively.The experimental group showed decrease in migration area compared to the NC group,and these differences were statistically significant(P＜0.05,P＜0.001).Compared to the NC group,the migration and invasion cell numbers in the experimental group(98.00±13.49 and 88.00±5.72)were significantly reduced to(48.00±2.16 and 49.00±2.94),and these differences were statistically significant(P＜0.01,P＜0.001).Conclusion Interference of SNCG expression can significantly reduce the migration and invasion ability of oral squamous cell carcinoma cells.
