Effects of TAS-102 combined with oxaliplatin on proliferation,migration,invasion and apoptosis of hepatocellular carcinoma SNU-449 cells
10.13699/j.cnki.1001-6821.2024.09.005
- VernacularTitle:TAS-102联合奥沙利铂对肝细胞癌SNU-449细胞增殖、迁移和侵袭以及凋亡的影响
- Author:
Yang LIU
1
;
Lu TAO
;
Yu-Mei LI
;
Yu-Fo CHEN
;
Rui WANG
Author Information
1. 蚌埠医学院第一附属医院肿瘤内科,安徽蚌埠 233004
- Keywords:
trifluridine-tipiracil hydrochloride tablets;
oxaliplatin;
hepatocellular carcinoma;
proliferation;
migration;
invasion;
apoptosis
- From:
The Chinese Journal of Clinical Pharmacology
2024;40(9):1262-1266
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of trifluridine-tipiracil hydrochloride tablets(TAS-102)combined with oxaliplatin(L-OHP)on proliferation,migration,invasion and apoptosis of hepatocellular carcinoma SNU-449 cells and its possible mechanism.Methods SNU-449 cells were divided into control group(without drugs),TAS-102 group(2 μg·mL-1),oxaliplatin group(1.2 μg·mL-1)and combination group(2 μg·mL-1 TAS-102+1.2 μg·mL-1 L-OHP).Cell counting kit-8 assay was used to detect cell proliferation ability;Transwell assay was used to detect cell migration and invasion;the apoptosis rate was analyzed by flow cytometry;the expressions of B-cell lymphoma-2(Bcl-2),Bel-2-associated X(Bax),E-cadherin and Vimentin were detected using Western blotting.Results Survival rate of cells in control group,TAS-102 group,oxaliplatin group and combination group were(100.00±2.98)％,(80.26±5.55)％,(78.49±5.76)％and(47.82±4.26)％,respectively;the number of cell migration were 436.83±27.50,228.17±15.87,171.67±19.49 and 113.83±11.34,respectively;the number of cell invasion were 332.67±19.95,205.33±13.65,219.67±14.49 and 116.83±11.30,respectively;the apoptosis rates were(2.32±0.07)％,(6.74±0.24)％,(6.37±0.18)％and(10.67±0.41)％,respectively;the relative expression levels of Bax protein were 0.28±0.01,0.53±0.03,0.75±0.03 and 1.11±0.03,respectively;the relative expression levels of Bel-2 protein were 0.65±0.03,0.32±0.02,0.23±0.02 and 0.13±0.01,respectively;the relative expression levels of E-cadherin were 0.15±0.01,0.24±0.02,0.35±0.02 and 0.64±0.02,respectively;the relative expression levels of Vimentin were 0.65±0.03,0.27±0.01,0.34±0.02 and 0.18±0.01,respectively.Comparison of each treatment group with the control group and comparison of the combination group with monotherapy group,the above indexes were statistically significant(all P＜0.05).Conclusion The synergistic effect of TAS-102 and oxaliplatin enhanced the inhibitionon proliferation,migration,and invasion,and promoted cell apoptosis of SNU-449 cells,and the mechanism may be related to the promotion of Bax and E-cadherin proteins and the inhibition of Bel-2 and Vimentin proteins.
