Determination of aspirin and salicylic acid of children with Kawasaki disease by HPLC-IDMS
10.13699/j.cnki.1001-6821.2024.08.024
- VernacularTitle:用高效液相色谱-同位素稀释质谱法测定川崎病患儿血浆中阿司匹林和水杨酸的药物浓度
- Author:
Ying-Hua MA
1
;
Yi-Le ZHAO
;
Ya-Bin QIN
;
Ying-Qian ZHANG
Author Information
1. 河北省儿童医院药学部,河北石家庄 050031
- Keywords:
aspirin;
salicylic acid;
high performance liquid chromatography-isotope dilution mass spectrometry;
isotope internal standards;
therapeutic drug monitoring
- From:
The Chinese Journal of Clinical Pharmacology
2024;40(8):1203-1207
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a method for determination of aspirin(ASP)and salicylic acid(SA)in plasma of children with kawasaki disease by high performance liquid chromatography-isotope dilution mass spectrometry(HPLC-IDMS).Methods The samples were pre-treated by precipitation protein method with organic solvent.ASP-D4 and SA-D4 were used as isotope internal standards.Chromatographic column:Phenomenex Kinetex? XB C18(2.6 mm × 50.0 mm,3.0μm);flow phase:0.1％formic acid-water(A)and acetonitrile(B),gradient elution;flow speed:0.5 mL·min-1;injection volume:6 μL.The detection method of mass spectrometry was negative ion mode,multireaction monitoring mode for quantitative analysis.Results The linear range of aspirin was 1.02-4 000 ng·mL-1(r2=0.995 4),and lower limit of quantification(LLOQ)was 1.02 ng·mL-1.The linear range of salicylic acid was 1.28-5 000 ng·mL-1(r2=0.998 5),and LLOQ was 1.28 ng·mL-1.Accuracy,precision,matrix effect and stability were in line with the provisions of Chinese Pharmacopoeia(2020 edition).Conclusion This study determined the blood concentration of aspirin and salicylic acid in children with HPLC-IDMS,which is rapid,simple,stable,economical,and high specificity.It can be applied to therapeutic drug monitoring of aspirin and salicylic acid in clinical children with kawasaki disease.
