Curcumin regulates the proliferation inhibition of gastrointestinal stromal tumor cells by inhibiting the inflammatory factor IL-6
10.13699/j.cnki.1001-6821.2024.08.015
- VernacularTitle:姜黄素通过抑制炎症因子IL-6调控胃肠道间质瘤细胞的增殖抑制的研究
- Author:
Yan CHEN
1
,
2
;
Yu-Ke LI
;
Ru-Jing WANG
;
Hong-Tao XIAO
;
San-Jun SHI
Author Information
1. 成都中医药大学药学院,四川成都 611137
2. 四川省肿瘤临床医学研究中心、四川省肿瘤医院研究所、四川省癌症防治中心、电子科技大学附属肿瘤医院药学部,四川成都 610041
- Keywords:
Curcumin;
inflammation;
interleukin-6;
gastrointestinal stromal tumor;
cell proliferation
- From:
The Chinese Journal of Clinical Pharmacology
2024;40(8):1160-1164
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate whether curcumin is a potential drug for the treatment of gastrointestinal stromal tumors(GIST).Methods The differential genes of imatinib-resistant cells and non-resistant cells were analyzed by cell transcriptology.The antitumor activity of curcumin was verified by cell counting kit-8(CCK-8)method,and the concentration of Curcumin ranged from 5 to 80 μg·mL-1for GIST-T1 and GIST-T1/IMR cells.20 μg·mL-1 Curcumin as the experimental group,phosphate buffered solution as the control group.The contents of interleukin-6(IL-6),reactive oxygen species(ROS)and nitric oxide(NO)were measured by enzyme linked immunosorbent assay.The cell cycle changes were analyzed by flow cytometry.Results Using non-resistant cells as a contrast,the results showed that there were 1 300 up-regulated genes and 1 609 down-regulated genes in imatinib-resistant cells.The 50％inhibiting concentration values of Curcumin on GIST-T1 and GIST-T1/IMR cells were(15.33±1.36)and(10.49±2.12)μg·mL-1,respectively.In GIST-T1 cells,the IL-6 levels in experimental group and control group were(3.45±0.01)and(5.64±0.42)pg·mL-1;the ROS levels were(2 841.42±81.83)and(4 174.32±439.12)pg·mL-1;the iNOS levels were(7.02±0.08)and(8.08±0.03)μmol·L-1,respectively.In GIST-T1/IMR cells,the IL-6 levels in experimental group and control group were(2.47±0.30)and(6.30±0.01)pg·mL-1;the ROS levels were(4 706.40±146.71)and(8 254.34±342.35)pg·mL-1;the iNOS levels were(6.42±0.09)and(7.29±0.04)μmol·L-1,respectively.Among the 2 cells,the differences of above indicators were statistically significant between the experimental group and the control group(P＜0.05,P＜0.01).Curcumin blocked the cell cycle of GIST-T1 and GIST-T1/IMR in G1 phase,further shortens S phase and G2 phase.Conclusion Curcumin can inhibit the secretion of inflammation and regulate the proliferation of GIST.
