Simultaneous determination of polymyxin B and tigecycline concentrations in rat plasma by LC-MS/MS method
10.13699/j.cnki.1001-6821.2024.07.024
- VernacularTitle:用LC-MS/MS法同时测定大鼠血浆中多黏菌素B和替加环素的浓度
- Author:
Zhu-Hang HAN
1
;
Yue LIU
;
Wen-Li LI
;
Tian-Lun ZHENG
;
Xiu-Ling YANG
Author Information
1. 河北医科大学第二医院药学部,河北石家庄 050000
- Keywords:
polymyxin B;
tigecycline;
liquid chromatography tandem mass spectrometry;
pharmacokinetics;
bioanalysis
- From:
The Chinese Journal of Clinical Pharmacology
2024;40(7):1049-1053
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a liquid chromatography tandem mass spectrometry(LC-MS/MS)method for the determination of polymyxin B and tigecycline in rat plasma and to study the pharmacokinetic profile in rats.Methods Rat plasma was treated with 3％trichloroacetic acid-methanol solution(50∶50)for protein precipitation on a Symmetry C18(150.0 mm × 4.6 mm,3.5 μm)column,with mobile phase:0.1％formic acid in water-0.1％formic acid in acetonitrile at a flow rate of 0.6 mL·min-1,the column temperature was 40 ℃,and the ionization source was electrospray ionization,positive ion detection mode:multiple reaction detection.The method was investigated for its specificity,standard curve and lower limit of quantification,precision and recovery,stability and reproducibility.Results The linear range of tigecycline was 25-2 500 ng·mL-1,the lower limit of quantification was 25 ng·mL-1,and the extraction recovery was 95.89％-107.90％;the linear range of polymyxin B,was 82-8 200 ng·mL-1,the lower limit of quantification was 80 ng·mL-1,and the extraction recovery was 93.84％-97.70％;the linear range of polymyxin B2 was 9-900 ng·mL-1,the lower limit of quantification was 9 ng·mL-1,the extraction recovery was 96.41％-104.80％;the intra-day and inter-day relative standard deviations of each substance were 96.41％-104.80％.The linear range was 9-900 ng·mL-1,the lower limit of quantification was 9 ng·mL-1,and the extraction recoveries were 96.41％-104.80％.The intra-day and inter-day relative standard deviations of each substance were less than 10％,and the stability and reproducibility were good.Conclusion This method is simple,sensitive,and has a short analytical time,and is suitable for the determination of the blood concentration of polymyxin B and tigecycline in rat plasma as well as for pharmacokinetic studies.
