Effects of emodin modulation of the HIF-1α/VEGF pathway on vascular endothelial cells damage in diabetic macroangiopathy rats
10.13699/j.cnki.1001-6821.2024.06.016
- VernacularTitle:大黄素调节HIF-1α/VEGF通路对糖尿病大血管病变大鼠血管内皮细胞损伤的影响
- Author:
Qiu-Xiao ZHU
1
;
Hui-Yao HAO
;
Zi-Bo LIU
;
Ming GAO
;
Fang ZHANG
;
Jing ZHOU
;
Zhi-Hua HAO
;
Li-Hui ZHANG
;
Yong-Mei HAO
Author Information
1. 河北医科大学第二医院 内分泌科,河北石家庄 050000
- Keywords:
emodin;
hypoxia inducible factor 1α/vascular endothelial growth factor signal pathway;
diabetes macroangiopathy;
inflammation;
oxidative stress
- From:
The Chinese Journal of Clinical Pharmacology
2024;40(6):859-863
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the impact of emodin(EM)on vascular endothelial cell injury in rats with diabetes macroangiopathy by regulating hypoxia inducible factor-1α(HIF-1α)/vascular endothelial growth factor(VEGF)signaling pathway.Methods SD rats were divided into blank group and modeling group,the rats in the modeling group were fed with high fat and high sugar combined with N-nitro-L-arginine methyl ester to build the diabetes macroangiopathy model,and the blank group was fed with ordinary diet.The vascular endothelial cells successfully isolated from the thoracic aorta of rats in blank group and modeling group were named control group and model group,respectively.The vascular endothelial cells in the modeling group were divided into model group,dimethyloxallyl glycine(DMOG)group(10 μmol·L-1DMOG),combined group(80 mg·L-1EM+10 μmol·L-1 DMOG)and experimental-L,-M,-H groups(20,40,80 mg·L-1 EM).The apoptosis of rat vascular endothelial cells was detected by flow cytometry;Western blot was applied to detect the expression of HIF-1αand VEGF proteins in rat vascular endothelial cells.Results The apoptosis rates of vascular endothelial cells in experimental-M,-H groups,DMOG group,combined group,model group and control group were(10.18±0.36)％,(6.28±0.20)％,(24.96±1.18)％,(12.36±0.49)％,(18.76±0.68)％and(4.59±0.26)％;HIF-1α protein levels were 0.96±0.07,0.78±0.06,2.03±0.12,1.05±0.13,1.58±0.12 and 0.69±0.05;VEGF protein levels were 0.59±0.05,0.23±0.02,0.98±0.06,0.63±0.04,0.86±0.07 and 0.11±0.01.The above indexes in the model group were compared with the control,DMOG,experimental-M and experimental-H groups,and the above indexes in the combined group were compared with the experimental-H group,and the differences were statistically significant(all P＜0.05).Conclusion EM may inhibit HIF-1α/VEGF pathway to improve vascular endothelial cell injury in rats with diabetes macroangiopathy.
