Effects of curcumol on iron death and epithelial-mesenchymal transition in hepatic stellate cells
10.13699/j.cnki.1001-6821.2024.04.013
- VernacularTitle:莪术醇对肝星状细胞铁死亡和上皮间质转换作用的研究
- Author:
Lei WANG
1
,
2
;
Jin-Biao HUANG
;
Yan-Qing HUANG
;
Ze-Yu WANG
;
Jia-Hui WANG
;
Yang ZHENG
;
Wei-Sheng LUO
;
Tie-Jian ZHAO
Author Information
1. 广西中医药大学研究生学院,广西壮族自治区南宁 530222
2. 广西中医药大学赛恩斯新医药学院,广西壮族自治区南宁 530222
- Keywords:
curcumol;
hepatic stellate cell;
ferroptosis;
epithelial-mesenchymal transition;
liver fibrosis
- From:
The Chinese Journal of Clinical Pharmacology
2024;40(4):539-543
- CountryChina
- Language:Chinese
-
Abstract:
Objective To elucidate the effect of curcumol on hepatic stellate cell iron death and epithelial-mesenchymal transition(EMT),and to investigate the molecular mechanism of its anti-liver fibrosis effect.Methods A model of hepatic stellate cell activation was constructed using normal cultured hepatic stellate cells in vitro,and the cells were divided into blank group and experimental-L,-M,-H groups.The blank group was given DMEM complete culture solution for normal culture;the experimental-L,-M,-H groups were given DMEM complete culture solution containing 12.5,25.0 and 50.0 mg·L-1 curcumol for 48 h of intervention.The effects of curcumol on the proliferation of hepatic stellate cells was observed by CCK-8.The expression levels of glutathione peroxidase 4(GPX4)and solute carrier family 7 member 11(SLC7A11)were detected by Western blot.The expression levels of E-cadherin and N-cadherin were detected by immunofluorescence.Results The cell proliferation rates of the experimental-M,-H groups and blank group were(68.97±5.61)％,(61.91±4.40)％and(118.07±10.01)％;the relative expression levels of GPX4 were 0.37±0.04,0.28±0.03 and 0.58±0.05;the relative expression levels of SLC7A11 were 0.38±0.04,0.28±0.03 and 0.60±0.05;E-cadherin levels were 6.76±1.09,9.57±1.73 and 2.05±0.72;N-cadherin levels were 5.66±0.66,3.44±0.78 and 10.37±0.66.The differences of above indicators were statistically significant between the blank group and the experimental-M,-H groups(P＜0.05,P＜0.01).Conclusion Curcumol promotes iron death in hepatic stellate cells,thereby inhibiting hepatic stellate cell EMT,which may be its molecular mechanism to prevent and treat liver fibrosis.
