Protective effects of dexmedetomidine on oxygen glucose deprivation/reperfusion cardiomyocyte injury by regulating iron metabolism
10.13699/j.cnki.1001-6821.2024.03.011
- VernacularTitle:右美托咪定调控铁代谢对氧糖剥夺/再灌注心肌细胞损伤的保护作用
- Author:
Wei DONG
1
;
Juan WANG
;
Wei ZHAO
;
Chen BEN
Author Information
1. 淮北矿工总医院麻醉科,安徽淮北 235000
- Keywords:
dexmedetomidine;
iron metabolism;
oxygen-glucose deprivation/reperfusion;
cardiomyocytes;
oxidative stress
- From:
The Chinese Journal of Clinical Pharmacology
2024;40(3):354-357
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of dexmedetomide on oxygen glucose deprivation/reperfusion(OGD/R)myocardial cell injury.Methods H9c2 cardiomyocytes were cultured in vitro and divided into control group(routine culture),model group(OGD/R treatment),experimental-L,-M,-H groups(treated with 1,5 and 10 μmoL·L-1 dexmedetomidine after oxygen glucose deprivation/reperfusion).Apoptosis was detected by flow cytometry;the levels of glutathione peroxidase(GSH-PX),superoxide dismutase(SOD)and lactate dehydrogenase(LDH)were detected by enzyme-linked immunosorbent assay;and Fe2+levels were detected by FerroOrange fluorescent probe.Results The apoptosis rates of control group,model group,experimental-L,-M,-H groups were(4.11±0.35)％,(27.57±2.36)％,(20.52±1.43)％,(14.17±1.55)％,(8.47±0.89)％,respectively;GSH-PX were(26.75±3.14),(126.68±11.42),(98.74±9.23),(73.15±8.02),(45.85±4.80)mU·mL-1,respectively;SOD were(1.59±0.11),(7.52±1.13),(6.12±0.74),(4.97±0.54),(2.35±0.69)ng·mL-1,respectively;LDH were(13.42±1.53),(152.15±18.94),(103.15±12.45),(64.59±7.81),(27.85±3.42)ng·mL-1,respectively;Fe2+were level(20.26±2.93),(63.85±6.44),(52.17±4.58),(40.15±4.12),(27.48±3.08)mmol·L-1,respectively.There were statistically significant differences between control group and model group(all P＜0.05);the model group was compared with experimental-L,-M,-H groups,and the differences were statistically significant(all P＜0.05).Conclusion Dexmedetomidine can alleviate myocardial cell injury induced by OGD/R by regulating iron metabolism.
