Effect of fucoidan on kidney injury in mice with systemic lupus erythematosus
10.13699/j.cnki.1001-6821.2023.24.020
- VernacularTitle:岩藻多糖对系统性红斑狼疮小鼠肾损伤的影响
- Author:
Rui-Ling MENG
1
;
Hui-Li LI
;
Zhi-Cheng ZHANG
;
Yin-Huai XU
Author Information
1. 濮阳医学高等专科学校医学技术系,河南濮阳 457000
- Keywords:
fucoidan;
systemic lupus erythematosus;
kidney injury;
inflammatory response;
Nod-like receptor protein 3 inflammasome
- From:
The Chinese Journal of Clinical Pharmacology
2023;39(24):3638-3642
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the protective effects of fucoidan on renal injury in systemic lupus erythematosus(SLE)mice and its mechanism.Methods Female MRL/lpr mice were randomly divided into model group,low dose experimental group,high dose experimental group and positive control group,with 10 mice in each group.Another 10 female MRL/MpJ mice were selected as control group.Mice in low dose experimental and high dose experimental groups were intragastrically given 150 and 300 mg·kg-1 fucoidan,mice in positive control group were intragastrically given 5 mg·kg-1 of prednisone,mice in control group and model group were intragastrically given the same amount of normal saline.After 4 weeks of administration,blood and organs of thymus,spleen and kidney were collected and stored for later use.The histopathological changes of kidney were observed by hematoxylin-eosin(HE)staining.Serum blood urea nitrogen(BUN)and serum creatinine(Scr)levels were detected by the kit.Serum inflammatory factors were detected by kit method.The expression levels of Nod-like receptor protein 3(NLRP3)and caspase-1 in renal tissues were determined by immunohistochemistry and Western blot.Results The spleen index of mice in control group,model group,low dose experimental group,high dose experimental group and positive control group were 4.56±0.24,2.41±0.38,2.91±0.23,3.70±0.35 and 3.12±0.51;BUN levels were(4.02±0.57),(11.26±1.60),(9.38±0.63),(7.73±0.42)and(7.09±0.70)mmol·L-1;IL-18 levels were(15.11±1.60),(62.45±7.45),(42.64±5.76),(23.88±2.96)and(30.65±2.77)ng·L-1,respectively.The above indicators,model group compared with control group;low dose experimental group,high dose experimental group and positive control group were compared with model group,respectively;high dose experimental group compared with low dose experimental group,the differences were all statistically significant(all P＜0.05).Conclusion Fucoidan can improve the histopathological changes of kidney in SLE mice,regulate immune function,control inflammation and improve renal function,which may be related to the inhibition of NLRP3 inflammatory bodies.
