The role and mechanism of LncRNA SOCS2-AS1 in the proliferation,invasion and migration of gastric cancer cells by regulating Hippo-YAP pathway
10.13699/j.cnki.1001-6821.2023.24.016
- VernacularTitle:长链非编码RNA SOCS2-AS1通过Hippo-YAP信号通路调控胃癌细胞增殖和侵袭迁移的机制研究
- Author:
Wen-Yao LÜ
1
;
Lin LI
;
Chong-An XU
Author Information
1. 中国医科大学附属第四医院肿瘤内科,辽宁沈阳 110000
- Keywords:
long non-coding RNA SOCS2-AS1;
gastric cancer;
Hippo-Yes associated protein signaling pathway;
proliferation;
invasion
- From:
The Chinese Journal of Clinical Pharmacology
2023;39(24):3618-3622
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effect of long non-coding RNA(lncRNA)SOCS2-AS1 on the proliferation,invasion and migration of gastric cancer cells and its potential mechanism.Methods 40 cases of gastric cancer tissues and 40 cases of adjacent tissues were selected as experimental specimens.Real-time quantitative polymerase chain reaction(RT-qPCR)was used to detect the expression level of lncRNA SOCS2-AS1 in gastric cancer tissues and adjacent tissues.Human gastric cancer SGC-7901 cells were divided into vector group,pcDNA-SOCS2-AS1 group,NC-siRNA group,SOCS2-AS1-siRNA group,pcDNA-SOCS2-AS1+vector group and pcDNA-SOCS2-AS1+pcDNA-YAP1 group.Vector group,pcDNA-SOCS2-AS1 group,NC-siRNA group and SOCS2-AS1 siRNA group were transfected with vector,pcDNA-SOCS2-AS1,NC-siRNA and SOCS2-AS 1 siRNA plasmids,respectively;the pcDNA-SOCS2-AS1+vector group were transfected with the pcDNA-SOCS2-AS1+vector plasmids;the pcDNA-SOCS2-AS1+pcDNA-YAP1 group were transfected with both pcDNA-SOCS2-AS1+pcDNA-YAP1 plasmids simultaneously.RT-qPCR was used to detect the expression level of lncRNA SOCS2-AS1 in cells;Western blotting was used to detect the expression of Hippo-YAP pathway-related proteins LATS1 and YAP1 in gastric cancer cells;EDU staining was used to detect the proliferation of gastric cancer cells;Transwell assay was used to detect cell migration and invasion.Results The relative expression levels of SOCS2-AS1 in gastric cancer tissues,adjacent tissues,normal gastric epithelial cells GES-1 and gastric cancer cells SGC-7901 were 1.00±0.18,0.38±0.09,1.03±0.21 and 0.42±0.11.Compared with the adjacent tissues and normal gastric epithelial cell line GES-1,SOCS2-AS1 was significantly down-regulated in gastric cancer tissues and gastric cancer cell line SGC-7901,and the differences were statistically significant(P＜0.05).The cell proliferation rates in the vector group,pcDNA-SOCS2-AS1 group,NC-siRNA group and SOCS2-AS1-siRNA group were(36.23±2.53)％,(18.15±1.04)％,(35.83±2.15)％and(56.11±6.57)％;the invasive cell numbers were 168.15±19.11,54.36±6.27,124.47±14.53 and 238.62±21.34;the numbers of migrating cells were 194.22±16.33,91.61±8.47,148.14±16.25 and 279.31±24.68;the relative expression levels of LATS1 protein were 0.93±0.08,2.86±0.21,0.96±0.09 and 0.31±0.03;the relative expression levels of YAP1 protein were 0.95±0.11,0.28±0.04,0.92±0.12 and 3.16±0.28;the pcDNA-SOCS2-AS1 group was significantly lower than the vector group,and the SOCS2-AS1-siRNA group was significantly higher than the NC-siRNA group,and the differences were statistically significant(all P＜0.05).The cell proliferation rates of pcDNA-SOCS2-AS1+vector group and pcDNA-SOCS2-AS1+pcDNA-YAP1 group were(33.62±5.73)％and(88.46±9.17)％;the number of invasive cells were 53.61±6.44 and 131.73±15.29;the number of migrating cells were 68.35±7.63 and 159.81±16.47,the differences were statistically significant(all P＜0.05).Conclusion LncRNA SOCS2-AS1 inhibits the proliferation,invasion and migration of gastric cancer cells by activating the Hippo-YAP signaling pathway.
