Exploration of the intervention effect and action mechanism of ginkgo flavonoids for non-alcoholic fatty liver disease mice
10.13699/j.cnki.1001-6821.2019.03.020
- VernacularTitle:银杏黄酮对非酒精性脂肪肝小鼠的影响及其机制
- Author:
Yu-Xiu MA
1
;
Yi-Ying KOU
Author Information
1. 青海省第四人民医院 肝病科
- Keywords:
non-alcoholic fatty liver disease;
ginkgo flavonoids;
lipid metabolism;
liver function;
nuclear transcription factor-κB p65
- From:
The Chinese Journal of Clinical Pharmacology
2019;35(8):795-797
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effect of ginkgo flavonoids on mice with non-alcoholic fatty liver disease (NAFLD). Methods A total of 60 ICR male mice were given high fat diet to induce NAFLD,and then assigned to model group and experimental-L/-M/-H groups,with 15 cases in each group. Fifteen ICR male mice were assigned to control group to receive regular diet. The experimental-L/-M/-H groups were given oral administration of 75,150,300 mg·kg-1 ginkgo flavonoids once daily for 8 weeks,while the model group and control group received equal amount 0. 9％NaCl. At 8 weeks after treatment,the liver lipid metabolism indexes and serum liver function indexes content were detected; the expression of liver tissue nuclear transcription factor - κB p65 (NF-κBp65) protein of mice were detected by western blot. Results The serum glutamic pyruvic transaminase (GPT) levels in control group,model group and experimental-L/-M/-H groups were (41. 25 ± 5. 21) ,(82. 19 ± 5. 13) ,(76. 53 ± 4. 89) ,(60. 02 ± 4. 83) ,(53. 89 ± 4. 52) U·L-1,respectively; the glutamic oxaloacetic transaminase (GOT) levels were (101. 33 ± 18. 76) ,(179. 84 ± 18. 39) ,(161. 28 ± 16. 48) ,(149. 06 ± 16. 23) ,(132. 17 ± 16. 05) U·L-1, respectively; the liver tissue total cholesterol (TC) contents were(1. 31 ± 0. 24) ,(3. 96 ± 0. 22) ,(3. 24 ± 0. 21) ,(2. 90 ± 0. 19) ,(2. 54 ± 0. 16) mmol·g-1, respectively; the triglyceride(TG) contents were (1. 39 ± 0. 23) ,(2. 71 ± 0. 21) ,(2. 32 ± 0. 20) ,(1. 94 ± 0. 18) ,(1. 76 ± 0. 16) mmol·g-1, respectively; the relative expression of live tissue NF-κBp65 protein were 0. 16 ± 0. 08,0. 89 ± 0. 12,0. 82 ± 0. 09,0. 31 ± 0. 07,0. 25 ± 0. 04,respectively. There were statistically significant differences between the model group and the control group/experimental-L/-M/-H group,respectively (P < 0. 05). Conclusion Ginkgo flavonoids can inhibit NAFLD mice liver fat degeneration,recover the liver lipid metabolism and liver function,the action mechanism maybe related to the down-regulation of the expression liver tissue NF-κBp65 protein.
