Protective effects of glutamine on intestinal ischemia reperfusion injury in rats and its mechanism
10.13699/j.cnki.1001-6821.2019.08.015
- VernacularTitle:谷氨酰胺对大鼠肠缺血再灌注损伤的保护作用及相关机制
- Author:
Jin HUA
1
;
Qi-Bao DAI
Author Information
1. 福建医科大学 附属第一医院 胃肠外科二区
- Keywords:
glutamine;
intestinal ischemia-reperfusion injury;
pathological morphology of intestinal tissue;
nuclear factor kappa-B;
peroxisome proliferator-activated receptor gamma
- From:
The Chinese Journal of Clinical Pharmacology
2019;35(8):776-779
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of glutamine on intestinal ischemia reperfusion injury(IR) in rats and the related mechanisms. Methods According to the weight, rats were divided into five groups: control group,experimental-A group,experimental-B group,experimental- C group and experimental-D group. The A group and B group were preoperatively given glutamine (1. 5 mg·kg-1) through tail vein injection and intragastric administration,respectively. The control group was preoperatively administered 1 mL 0. 9％ sodium chloride was given for the rat each day by tail vein injection. The experimental-C group and experimental-D group were given glutamine(1. 5 mg·kg-1) through injection of superior mesenteric vessel and intestinal cavity,respectively,after open abdomen,once a day for 4 days. Meanwhile,the model of intestinal IR was established by clamping the superior mesenteric vessel. Intestinal tissues and blood samples were taken after ischemia for 50 minutes,reperfusion for 1 h and 12 h. The changes of tumor necrosis factor-alpha(TNF-α) content in blood samples were detected by enzyme-linked immunosorbent assay. RT-PCR were used to detect nuclear factor kappa-B(NF-κB) and peroxisome proliferator-activated receptor gamma(PPAR-γ) mRNA in intestinal specimens of rats with intestinal IR. Western-blot were used to detect NF-κB and PPAR-γ protein expression. Results After intestinal reperfusion for 12 h, serum TNF-α levels in control,experimental-A,experimental-B,experimental-C and experimental-D groups were respectively(419. 04 ± 10. 55) ,(311. 77 ± 9. 81) ,(224. 53 ± 3. 36) ,(318. 77 ± 15. 64) and (436. 20 ± 22. 50) ng·L-1; comparison between experimental-A group and experimental-B group with control group, the difference was statistically significant (all P < 0. 05); comparison between experimental-A group and experimental-B group with experimental- D group,the difference was statistically significant (all P < 0. 05). The expression levels of NF-κB mRNA in the five groups were 1,0. 51 ± 0. 03,0. 61 ± 0. 07,0. 62 ± 0. 09 and 0. 92 ± 0. 05,respectively; meanwhile,the expression levels of PPAR-γ mRNA in the five groups were 1,2. 91 ± 0. 22,2. 52 ± 0. 24,1. 48 ± 0. 12 and1. 39 ± 0. 13,respectively; comparison between experimental-A group with control group and experimental-D group,the difference was statistically significant (all P < 0. 05). The trend of protein results is consistent with that of gene. The trend of 1 h results is consistent with that of 12 h. Conclusion Glutamine can play a protective role to intestinal IR rats via a mechanism of reducing the activity of NF-κB and increasing the activity of PPAR-γ.
