Determination of cryptotanshinone and tanshinone Ⅱ A in human plasma by LC-MS/MS
10.13699/j.cnki.1001-6821.2019.07.026
- VernacularTitle:LC-MS/MS法测定人血浆中隐丹参酮及丹参酮ⅡA的含量
- Author:
Xue-Ning ZHANG
1
;
Li-Guang DUAN
Author Information
1. 河北医科大学 第二医院 药学部
- Keywords:
cryptotanshinone;
tzanshinone ⅡA;
human plasma;
LC-MS/MS
- From:
The Chinese Journal of Clinical Pharmacology
2019;35(7):690-693
- CountryChina
- Language:Chinese
-
Abstract:
Objective To develop a LC-MS/MS method for the determination of cryptotanshinone and tanshinone Ⅱ A in human plasma.Methods The plasma samples were precipitated with methano1. After sample preparation, the samples were separated on a Symmetry C18 (150 mm × 4. 6 mm, 5 μm) column using acetonitrile and water (with 0. 1％formic acid) as mobile phase in gradient elution mode. Velocity of flow was 0. 1 m L·min-1. Column temperature was 35 ℃. Sample size was 10μL. The mass spectrometer was operated under the positive ion mode with the electrospray ionization source. And, the detection was performed by multiple reaction monitoring. The specificity, standard curve and lower limit of quantification, precision and recovery, stability of the method were investigated. Results The linear relationships for cryptotanshinone and tanshinone Ⅱ A were good in the concentration range of0. 50-100. 10 ng·m L-1 and 0. 51-101. 20 ng·m L-1, limit of quantitation were 0. 50 and 0. 51 ng·m L-1 and the intra-day and inter-day precision (RSD) were less than 8. 3％. The average recoveries for cryptotanshinone and tanshinone Ⅱ A at low, medium and high levels were greater than 88. 3％. Conclusion The analysis method was sensitive, simple and suitable enough to be applied for the determination of cryptotanshinone and tanshinone ⅡA in human plasma.
