Effect of oridonin on Th1 cell/Th2 cell balance in acute asthmatic mice
10.13699/j.cnki.1001-6821.2019.07.015
- VernacularTitle:冬凌草甲素对急性哮喘小鼠Th1细胞/Th2细胞平衡的影响
- Author:
Ye CHEN
1
;
Zhi-Li HU
;
Shou-Di HE
;
Xiao-Jun XIAO
;
Xin ZHAO
;
Bin FENG
;
Feng XU
Author Information
1. 深圳市南山区人民医院 儿科
- Keywords:
oridonin;
asthma;
Th1 cell;
Th2 cell;
immunomodulation
- From:
The Chinese Journal of Clinical Pharmacology
2019;35(7):651-653
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effect of oridonin on the Th1/Th2 balance induced by ovalbumin (OVA) in a mice model of asthma.Methods Balbc female mice were randomly divided into 4 groups:normal group, model group, and experimental-low, experimental-high groups, each group had 10 mice. The acute asthma model was induced by OVA. After the model was successfully established, the experimental group was injected with oridonin 20, 40 mg · kg-1, qd for 14 d. The normal group and the model group were given normal saline. The airway hyperresponsiveness of mice was detected by airway hyperresponsiveness instrument. Serum immunoglobulin E (IgE) and interleukin-4 (IL-4) were detected by enzyme-linked adsorption assay. The percentage of Th1, Th2 cells in spleen were detected by flow cytometry. Results The airway hyperresponsiveness values when the concentration of methacholine was 100 μg·m L-1 in model, experimental-low, experimental-high groups were 2. 99 ± 0. 56, 2. 72 ± 0. 68 and 2. 58 ± 0. 43, respectively.The serum IgE levels (OD values) in the three groups were 1. 13 ± 0. 17, 0. 76 ± 0. 21 and 0. 59 ± 0. 19, respectively. The serum IL-4 levels in the three groups were (66. 3 ± 2. 1) , (56. 7 ± 2. 5) and (42. 7 ± 2. 5) pg·m L-1. The Th1/Th2 in spleen of the three groups were 0. 67 ± 0. 04, 0. 99 ± 0. 04 and 1. 47 ± 0. 06, respectively.Comparison between experimental-low, experimental-high groups with model group, the differences of the factors were significant (P < 0. 05, P < 0. 01) . Conclusion Oridonin can reduce the level of serum IgE and regulate the differentiation of Th1/Th2, improvingthe inflammatory response of acute asthma model.
