Effects of rosuvastatin on inflammatory response pathway of focal cerebral ischemia reperfusion in rats
10.13699/j.cnki.1001-6821.2019.07.012
- VernacularTitle:瑞舒伐他汀对局灶性脑缺血再灌注大鼠炎性反应通路的影响
- Author:
Jie-Hua WANG
1
;
Guo-Qian LI
;
Zhu-Quan HONG
;
Shu-Jie GONG
;
Xiu-Xiu XU
;
Shu-Zeng CHEN
Author Information
1. 福建医科大学 附属泉州第一医院神经内科
- Keywords:
rosuvastatin;
cerebral ischemia-reperfusion;
Toll like receptor 4;
nuclear transcription factor kappa B p65
- From:
The Chinese Journal of Clinical Pharmacology
2019;35(7):640-642
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the role of rosuvastatin in inhibiting inflammatory response pathway in rats with focal cerebral ischemia-reperfusion (I/R) . Methods Rats were randomly assigned into three groups: sham operation group, model group and experimental group, each group had 8 rats. The rats in the experimental group were lavaged with rosuvastatin 5 mg·kg-1·d-1 for 10 consecutive days before the model setting-up. The middle cerebral artery occlusion I/R model was made by the modified Zea-Longa wire bolt method. At 24 h after reperfusion, the positive cells of Toll like receptor 4 (TLR4) and nuclear transcription factor kappa B p65 (NF-κB p65) were measured by immunohistochemistry; the levels of the TLR4, NF-κB p65 protein and the TLR4, NF-κB p65 mRNA were measured by Western blotting and reverse transcription PCR, respectively. Results The expressions of TLR4 positive cells in sham group, model group and experimental group were (12. 69 ± 2. 55) cells, (38. 05 ± 6. 81) cells and (27. 80 ± 5. 75) cells, respectively;the expressions of NF-κB p65 positive cells in the three groups were (14. 64 ± 2. 79) cells, (47. 27 ± 7. 40) cells and (34. 83 ± 5. 60) cells, respectively; the expressions of TLR4 protein (optical density, OD) in the three groups were0. 28 ± 0. 04, 0. 63 ± 0. 09, 0. 41 ± 0. 07; the expressions of NF-κB p65 protein (OD) in the three groups were0. 21 ± 0. 04, 0. 72 ± 0. 12, 0. 36 ± 0. 06. Comparison between sham operation group and model group showed the difference of the factors were statistically significant (all P < 0. 05) ; comparison between model group and experimental group displayed the difference of the factors were statistically significant (all P < 0. 05) . The trend of gene results is consistent with that of protein. Conclusion Rosuvastatin can inhibit TLR4-NF-κB signal transduction pathway and reduce several inflammatory reaction after focal cerebral I/R.
