Effect of Wuling Capsule on cognitive function of mice
10.13699/j.cnki.1001-6821.2018.20.008
- VernacularTitle:乌灵胶囊对小鼠认知功能的影响
- Author:
Xiao-Dong ZOU
1
;
Zheng-Zhong ZHANG
;
You-Geng CHENG
;
Zhong-Chun LI
;
Hai WANG
;
Zhi-Wei HU
Author Information
1. 浙江省立同德医院神经内科
- Keywords:
Wuling Capsule;
connexin 43;
cognitive function;
glial cell
- From:
The Chinese Journal of Clinical Pharmacology
2018;34(20):2421-2423
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of Wuling Capsule on cognitive function of Alzheimer disease model mice (5×FAD) and the mechanism of connexin 43 ( Cx43).Methods The 5xFAD mice were randomly divided into model group ( n =10 ) and experimental group (n=10),healthy mice as normal group (n=10).At 4 months old,the mice in the experimental group were given Wuling Capsule by gastrointesti-nal administration once a day at 9:00 am at a dose of 150 mg· kg-1· d-1 for 2 months, which was weighed to 40 mL in a 0.5% CMC-Na solu-tion.The normal group and model group received regular diet .The cog-nitive function of mice was evaluated by Water maze and Y maze .Immu-nohistochemical method was used to detect the expression of Cx 43 protein in the brain of mice.Results After the administration , the escape la-tency of the experimental group and model group on the third day after training was (58.0 ±1.3), (63.0 ±1.2) s, and the difference was sta-tistically significant ( P ＜0.05 ) . The quadrant retention time (32.0 ±1.5)s and cross-platform times (4.6 ±1.1) s of the experi-mental mouse platform were significantly higher than the model group (19.0 ±1.7), (1.9 ±1.2 ) s ( all P ＜0.05 ) .After two months of administration, the number of erroneous responses (3.9 ±0.5) times in the experimental group to passive stimuli for safe escape was significantly lower than that in the model group (6.3 ±1.6 ) times ( P＜0.05) .Conclusion Wuling Capsule can improve the cognitive function by increasing the secretion of Cx43 protein.
