Study on anti-inflammatory mechanism of volatile of Ramulus Cinnamom in acute pneumonia mice
10.13699/j.cnki.1001-6821.2018.20.006
- VernacularTitle:桂枝挥发油对急性肺炎小鼠的抗炎作用机制研究
- Author:
Chuan-Ju SUN
1
;
Yi-Qing ZHAO
;
Cai-Di ZHANG
;
Rong-Fang CHEN
Author Information
1. 无锡市第三人民医院 药学部
- Keywords:
acute pneumonia;
volatile of ramulus cinnamom;
anti-inflammatory effect;
transcriptional activator AP -1
- From:
The Chinese Journal of Clinical Pharmacology
2018;34(20):2413-2416
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the anti -inflammatory effect and mechanism of volatile of Ramulus Cinnamom ( VORC) on acute pneumonia mice.Methods Ninety ICR mice were randomly divided into 6 groups, normal group, model group, positive control group and low, middle, high dose experimental group (experimental -L,-M,-H group), each group with 15 mice, acute pneumonia model mice were established by intraperitoneal ( ip) injection of escherichia coli lipopolysac-charide (LPS), normal group ip the same amount of normal saline , experimental group immediately gavaged VORC at 0.03, 0.05, 0.10 mL· kg -1, positive control group given 5 mg· kg -1dexamethasone acetate , model group and nor-mal group given equal volume of normal saline , and continuously treated for 5 days.The levels of inflammatory cyto-kines in serum and the number of inflammatory cells in bronchoalceolar lavage fluid (BALF) were detected, morpholo-gy of lung tissue in mice was observed by hematoxylin -eosin (HE) staining, the expression of transcriptional activator protein 1 (AP-1) in lung tissue was detected by reverse transcriptase -polymerase chain reaction ( RT -PCR) . Results After treatment, the interleukin -6 (IL-6) content of normal group, model group, positive control group, experimental -L,-M,-H group after treatment were (87.49 ±7.69 ), ( 138.12 ±14.36 ), ( 109.15 ±4.58 ), (112.33 ±65.26),(108.22 ±55.38),(100.26 ±63.42)ng· L-1,respectively; the interleukin-1β(IL-1β) con-tent were (12.16 ±5.12),(59.48 ±10.35),(43.15 ±7.14),(50.12 ±6.33),(42.52 ±6.98),(26.15 ±5.34) ng· L-1,respectively; the tumor necrosis factor-α(TNF-α) content were (332.45 ±15.28),(455.15 ±27.25), (426.21 ±16.47),(432.15 ±15.66),(422.35 ±13.52),(402.15 ±12.05)ng· L-1,respectively.The number of BALF macrophages of normal group , model group, positive control group , experimental -L,-M,-H group after treatment were were ( 98.13 ± 10.28 ), ( 199.47 ± 24.35 ), ( 159.86 ± 16.47 ), ( 160.24 ± 15.48 ), (151.24 ±12.85 ),( 145.26 ±9.52 )×106/L, respectively; the number of neutrophils were ( 5.28 ±0.54 ), (61.45 ±5.16),(36.52 ±5.37 ),(42.15 ±6.33 ),(35.48 ±5.96 ),(23.58 ±6.25 )×106/L,respectively; the number of white blood cells were (105.48 ±8.69 ), ( 325.14 ±21.58 ), ( 224.13 ±16.24 ), ( 256.41 ±19.46 ), (233.15 ±15.46 ),(189.46 ±10.25 )×106/L,respectively.The levels of serum interleukin -6 (IL-6), interleukin-1β(IL-1β) and tumor necrosis factor -α(TNF -α) and macrophages, neutrophils and white blood cells in BALF of model group were significantly higher than those of the normal group , while experimental -L,-M,-H group and positive control group were markedly lower than those of the model group ( P＜0.01 ).The relative expression of c-jun mRNA in lung tissue of normal group, model group, positive control group, experimental-L,-M,-H group were 0.38 ±0.15 , 0.59 ±0.13, 0.41 ±0.15, 0.47 ±0.16, 0.46 ±0.12 , 0.39 ±0.15 respectively; the relative ex-pression of c-fos mRNA in lung tissue were 0.52 ±0.24 , 0.92 ±0.23 , 0.56 ±0.14 , 0.75 ±0.22 , 0.72 ±0.19 , 0.58 ±0.21 , respectively.The relative expression of c -jun and c-fos mRNA in lung tissue of model group were sig-nificantly higher than those of the normal group , while the relative expression of c -jun and c-fos mRNA in positive control group and experimental -L,-M,-H group were significantly lower than those in the model group ( P＜0.05 or P＜0.01).Conclusion VORC can reduce the inflammatory and pathological damage of lung tissue in acute pneu-monia mice, which may be achieved by inhibiting the activation of AP -1 signaling pathway.
