Effect of high mobility group chromosomal protein N2 ( HMGN2 ) on growth of bladder cancer cells and xenograft tumor in nude mice
10.13699/j.cnki.1001-6821.2018.20.003
- VernacularTitle:高迁移率蛋白N2对人膀胱移行细胞癌及裸鼠移植瘤的影响
- Author:
Ping JIANG
1
;
Jin-Qiao YUE
;
Jing LI
;
Zhen-Yu DONG
;
Xu YANG
;
Dong-Yu LEI
;
Gui-Xia WU
Author Information
1. 新疆医科大学基础医学院 生理教研室
- Keywords:
high mobility group chromosomal protein N2;
T24 cell;
xenograft tumor;
carcinoma of urinary bladder
- From:
The Chinese Journal of Clinical Pharmacology
2018;34(20):2402-2405
- CountryChina
- Language:Chinese
-
Abstract:
Objective The inhibitory effect of high mobility group chro-mosomal protein N2(HMGN2) on growth of human bladder cancer T24 cells and xenograft tumor in nude mice .Methods In vitro, using thia-zole blue method (MTT) to detect the effect of 0, 3, 5, 7, 9 μg· mL-1 HMGN2 on the activity of T24 cells.Flow cytometry was used to detect the inhibitory effect of 0, 1, 3, 5 μg· mL-1HMGN2 on T24 cells.The expressions of apoptotic proteins Bcl -2, caspase -3, p53 and Bax in cells were detected by Western blot .In vivo, xenograft tumor in nude mice was constructed and divided into blank group , control group and ex-perimental group.Phosphate buffer solution ( PBS ), cis -dichlorodia-mineplatinum (DDP) and HMGN2 were respectively injected around the tumor tissue.All nude mice were sacrificed on post -transplantation 20 d, tumor tissue was removed.The effects of necrosis and apoptosis was analyzed by Hematoxylin and eosin (HE) staining and TUNEL.Re-sults MTT results indicated that the growth of T24 cells was inhibited by 3, 5, 7, 9 μg· mL-1HMGN2, and the inhibition rate increased significantly with the increase of HMGN 2 concentra-tion and the incubation time.Flow cytometry results showed that the total apoptosis rate of 1, 3, 5 μg· mL-1HMGN2 in T24 cells were ( 13.18 ±0.83 )%, (25.79 ±1.22 )%, ( 31.48 ±1.82 )%, had significant difference with 0 μg· mL-1HMGN2 (P＜0.01 ).HE staining and TUNEL detection results of tumor tissues in nude mouser showed that compared with blank group , tumor tissues in experimental group and control group showed obvious necrosis and apoptosis.Conclusion HMGN2 may inhibit the proliferation of T24 cells and the growth of xenograft tumor in nude mice by promoting the necrosis of tumor tissue and inducing apoptosis .
