Determination of the content of cyclosporine in mice cornea by ultra-performance liquid chromatography-mass spectrometry
10.13699/j.cnki.1001-6821.2018.19.028
- VernacularTitle:超高效液相色谱-质谱法测定小鼠眼角膜中环孢素的含量
- Author:
Wen-Zhe PANG
1
;
Bin HAN
;
Qiang WANG
;
Yan-Xia GAO
;
Yi-Hua ZHANG
;
Xiao-Yan CUI
Author Information
1. 河北省药品检验研究院 抗生素室
- Keywords:
cyclosporine;
ultra -performance liquid chromatography -mass spectrometry;
cornea
- From:
The Chinese Journal of Clinical Pharmacology
2018;34(19):2342-2344
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a ultra performance liquid chroma-tography-mass spectrometry to determine the content of cyclosporine in mice.Methods Mobile phase was methanol -water (90∶10), chroma-tographic column was: Hypersill C18column (100 mm×2.1 mm, 1.9 μm) and internal standard substance : Cyclosporine A, ion mode of ESI +was applied to determine cyclosporine and multi -response monitoring mode was used (MRM, cyclosporine m/z: 1224.9 ).Results Working solution over the concentration range of 10 ～200 ng · mL-1 (R＝0.9986) worked well for the determination of cyclosporine in mice cornea homogenate.The lowest concentration was 3.3 ng· mL-1.The average extraction recovery rate of cyclosporine in mice cornea reached almost 80%(n＝6), and RSD was less than 12%.The intra-precision was less than 10.12%and the inter -precision was less than 11.75%. Conclusion The established method was sensitivity , specific, repea-table for cyclosporine determination in mice cornea .
