Inhibitory effect of zoledronic acid liposome on proliferation of oral epithelial carcinoma KB cells
10.13699/j.cnki.1001-6821.2018.19.019
- VernacularTitle:唑来膦酸脂质体抑制口腔上皮癌KB细胞增殖的研究
- Author:
Ying ZHANG
1
;
Ze-Biao ZHANG
;
Qiao-Feng CHEN
;
Yan-Ru CHEN
Author Information
1. 福建医科大学 附属泉州第一医院 口腔科
- Keywords:
zoledronic acid liposome;
oral epithelial carcinoma;
KB cells
- From:
The Chinese Journal of Clinical Pharmacology
2018;34(19):2309-2312
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the inhibitory effect of zoledronic acid liposome on proliferation of oral epithelial carcinoma KB cells .Methods The zoledronic acid liposome was prepared by reverse evaporation .The study was divided into blank , control and test groups.Blank group was given fresh medium.Control group received complete medium containing 10 μmol· L-1zoledronic acid.Test group was treated with complete me-dium containing 10 μmol· L-1zoledronic acid liposome.After interven-tion 24 h and 72 h, the survival rate of oral epithelial carcinoma KB cells was determined by MTT assay.Results The mean particle size was 100.72 nm, encapsulation rate was 83.56%, drug -loading rate was 5.21%, and average potential was -18.21 mV.In test and control groups, the survival rates after intervention 24 h were (44.87 ±7.23)%and (65.27 ±5.11 )%, the survival rates after intervention 48 h were (35.34 ±4.22 )%and (55.11 ±7.11 )%, the survival rates after inter-vention 72 h were (22.67 ±6.54 )%and (48.22 ±8.13 )%, the diffe-rences were statistically significant ( all P<0.001 ).Conclusion The zoledronic acid liposomes prepared by reverse evaporation can obtain uniform particle size distribution and good stability, meeting the requirements of pharmacology.The proliferation inhibitory effect of zoledronic acid liposome on oral epithelial carcinoma KB cells with was stronger than that of zoledronic acid free drug .
