Concentration determination of voriconazole in human plasma by HPLC-MS/MS
10.13699/j.cnki.1001-6821.2018.10.024
- VernacularTitle:HPLC-MS/MS法测定人血浆中伏立康唑浓度
- Author:
Yang LIU
1
;
Ting-Ting QIU
;
Chao ZHANG
Author Information
1. 北京大学第三医院药剂科
- Keywords:
voriconazole;
HPLC-MS/MS;
plasma concentration;
therapeutic drug monitoring
- From:
The Chinese Journal of Clinical Pharmacology
2018;34(10):1222-1225
- CountryChina
- Language:Chinese
-
Abstract:
Objective To develop a method for concentration determination of voriconazole in human plasma in order to apply to clinics.Methods HPLC-MS/MS method was adopted.Using d3-voriconazole as internal standard,the determination was performed on Agilent Eclipse Plus C18 column with mobile phase consisted of acetonitrile-water (containing 0.01 mol · L-1 ammonium formate) 55∶45 at flow rate of 0.3 mL · min-1 and column temperature of 40 ℃.The electrospray ion source,positive ionizing pattern and multiple reaction monitoring were used;the mass transition ion-pairs of voriconazole and internal standard were m/z 350.2→m/z 281.1 and m/z 353.2→m/z 284.1.Results The linear standard concentration range of voriconazole was 50-1.0 ×104 ng · mL-1(r =0.9997),and the lower limit of quantitation was 50 ng · mL-1.Precision (RSD) of inter-day and intra-day were lower than 5％.Method recovery was higher than 95％,and extraction recovery was higher than 85％.The steady state trough voriconazole plasma concentrations of 17 patients with hematologic malignancy were 0.38-8.10 μg · mL-1 determined by this method,of which 3 patients were outside of the recommended range.Conclusion The established assay method in this study is rapid,accurate and sensitive,and can be applied for the therapeutic drug monitoring of voriconazole.
