Study on acute toxicity and nephrotoxicity of antimicrobial peptide R9 in mice
10.13699/j.cnki.1001-6821.2018.10.023
- VernacularTitle:抗菌肽R9对小鼠急性毒性及肾毒性的研究
- Author:
Xiang-Ju CAI
1
;
Jun MA
;
Zhi-Xiao WANG
;
Run-Ze HE
;
Lan MA
Author Information
1. 甘肃中医药大学国家中医药管理局中医药科研三级实验室中药药理实验室
- Keywords:
antimicrobial peptide R9;
acute toxicity;
target organ;
nephrotoxicity;
positively correlated with dose
- From:
The Chinese Journal of Clinical Pharmacology
2018;34(10):1218-1221
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the acute toxicity and nephrotoxicity of antibacterial peptide R9 in mice.Methods Median lethal dose (LD50) test:Antimicrobial peptide R9 was intraperitoneally injected into mice.After 7 d of observation,the LD50 and 95 ％ confidence limits were calculated according to the Bliss method.Renal toxicity of R9 test:80 mice were randomly divided into 4 groups:the low,medium and high dose (6.7,10,15 mg · kg-1 R9) experimental groups and blank control group (distilled water) were injected intraperitoneally.The blood samples were collected on the 2nd day respectively from 10 mice,then blood indexes were tested.The serum were separated,then creatinine,blood urea nitrogen and total protein were measured.Kidneys and other organs were taken to calculate the organ coefficients.Results The LD50 of antimicrobial peptide R9 was 21.3 mg · kg-1,and its 95％ confidence limit was 19.1-23.5 mg · kg-1.On the second day after administration,WBC (4.07 × 109/L) and RBC (8.96 × 1012/L) in the high dose experimental group decreased compared with the blank control group,the differences were statistically significant difference (P ＜ 0.01,P ＜ 0.05).Creatinine (19.99 μmol · L-1) and urea nitrogen (8.07 mmol · L-1)increased with significant (P ＜0.05,P ＜ 0.01).The total protein (54.18 g · L-1) decreased with significant (P ＜0.05) and the kidney coefficient (13.40 mg· g-1) increased with significant (P ＜ 0.05).Conclusion A single intraperitoneal injection of antimicrobial peptide R9 can produce certain toxicity to mice,and the kidney was the main target organ.Nephrotoxicity was positively correlated with dose,and there was a trend of reversible improvement after withdrawal.
