Effect of seleno enriched Astragalus Saponin on the induced degenerated articular chondrocytes in vitro culture based upon Wnt/β-catenin signal pathway
10.13699/j.cnki.1001-6821.2018.10.021
- VernacularTitle:富硒黄芪皂苷对大鼠退变软骨细胞Wnt/β-catenin信号通路调控机制研究
- Author:
Chun-Lu YAN
1
;
Sheng-Hua LI
;
Fang-Yu AN
;
Hua JIN
;
Shi-Hua SUN
;
Lei ZHAO
;
Peng-Fei XIA
;
Peng-Yao LIU
;
Yong-Hua ZHANG
Author Information
1. 甘肃中医药大学教学实验实训中心
- Keywords:
seleno enriched Astragalus Saponin;
interleukin-1β;
degenerated chondrocytes;
Wnt signal pathway
- From:
The Chinese Journal of Clinical Pharmacology
2018;34(10):1210-1213
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effect of seleno enriched Astragalus Saponin on degenerated chondrocytes induced by interleukin-1β (IL-1β) in the Wnt/β-catenint signal pathway,to explore the mechanism of seleno enriched Astragalus Saponin on the intervention of cartilage degeneration.Methods Cells were divided into control group,model group and experimental group.Control group was given no drug treatment,degenerative chondrocytes were established by 10 ng · mL-1 IL-1β as model group and experimental group,25-300 mmol · L-1 seleno enriched Astragalus Saponin was intervened in experimental group.The tests of cell proliferation were analyzed by MTT,the gene expressions of DKK1,WISP1,Wnt1,β-catenin and LRP5 were checked by reverse transcription polymerase chain reaction(RT-PCR),the protein expressions of DKK1,WISP1,Wnt1,β-catenin and LRP5 were measured by Western blot.Results DKK1 gene expressions in control group,model group,100 mmol · L-1 experimental group were 1.00 ±0.00,0.37 ±0.24,0.79 ±0.26.WISP1 gene expressions in above groups were 1.00 ± 0.00,3.13 ± 0.33,1.35 ± 0.40,Wnt1 gene expressions in above groups were 1.00 ± 0.00,2.01 ±0.07,1.45 ± 0.33,β-catenin gene expressions in above groups were 1.00 ± 0.00,2.38 ± 0.30,1.32 ±0.17,LRP5 gene expressions in above groups were 1.00 ±0.00,1.70 ±0.17,1.25 ±0.23,DKK1 protein gray value in above groups were 0.52 ± 0.03,0.22 ± 0.02,0.36 ± 0.07,WISP1 protein gray value in above groups were 0.23 ± 0.04,0.58 ± 0.09,0.29 ± 0.03,Wntl protein gray value in above groups were 1.33 ± 0.06,2.78 ±0.15,1.54 ± 0.17,β-catenin protein gray value in above groups were 0.47 ± 0.02,0.80 ± 0.05,0.50 ±0.03,LRP5 protein gray value in above groups were 0.87 ±0.13,1.31 ±0.16,0.66 ±0.03.The cell proliferation abilities were accelerated by seleno enriched Astragalus Saponin intervention,but only 100 mmol · L-1 seleno enriched Astragalus Saponin intervention had significant difference at 48 h,meantime the expressions of gene and protein in WISP1,Wnt1,β-catenin and LRP5 were all decreased,the expressions of gene and protein in DKK1 were increased (P ＜ 0.05).Conclusion Seleno enriched Astragalus Saponin could markedly increase the proliferative activity of chondrocytes,this action was closely correlated with Wnt/β-catenin signal pathway.
