Effects of verbascoside on the proliferation and osteogenic differentiation of rat bone marrow stromal cells
10.13699/j.cnki.1001-6821.2018.10.020
- VernacularTitle:麦角甾苷对大鼠骨髓基质细胞增殖和成骨分化的影响研究
- Author:
Ou-Di SONG
1
;
Shu-Jian QIN
;
Shao-Long YU
;
Hui-Ying QU
;
Yuan TIAN
Author Information
1. 锦州医科大学基础学院
- Keywords:
verbascoside;
bone marrow stromal cells;
proliferation;
osteogenic differentiation
- From:
The Chinese Journal of Clinical Pharmacology
2018;34(10):1206-1209
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effects of verbascoside on the proliferation and osteogenic differentiation of rat bone marrow stromal cells (BMSCs) and its mechanism.Methods BMSCs were obtained and identified from rat bone marrow by density gradient centrifugation.BMSCs were randomly divided into high,medium,low dose test groups and control group,and treated with 100,50,25,0 μmol · L-1verbascoside.The cell counting kit-8 (CCK-8) method was used to evaluate the BMSCs proliferation at 24,48,72 h after administration the medication.The alkaline phosphatase(ALP) activities were detected at 24,48,96 h after medication.The ALP staining was performed after 72 h of medication.Western blot and real time-polymerase chain reaction (PCR) were used to detect the osteogenic related proteins and genes expression.Results After 72 h,the proliferation of BMSCs in high dose test group was 1.22 ± 0.05,lower than that in control group,which was 1.50 ± 0.09 (P ＜ 0.05).The ALP expression levels in high,medium and low dose test groups were higher than control group at 24,48,96 h.After 72 h,ALP staining showed that the color of culture dishes in high,medium and low dose test groups were deeper than that in control group.Western blot showed that osteogenic related proteins of bone morphogenetic protein-2 (BMP2),Smad1,Smad5,Smad8,Runx2,Osterix in high,medium and low dose test groups were higher than those in control group (P ＜0.05),but there was no significant difference among those three test groups (P ＞ 0.05).Real time-PCR showed that the osteogenic related genes of BMP2,Smad1,Smad5,Smad8,Runx2,Osterix expression levels in high,medium and low dose test groups were higher than those in control group (P ＜ 0.05).However,there was no significant difference among three test groups(P ＞ 0.05).Conclusion The verbascoside at different concentrations has no obvious effect on BMSCs proliferation and promote osteogenic differentiation of BMSCs through BMP2-Smads -Runx2-Osterix pathway.
