Effect of Sinisan on hippocampal neurons discharge amplitude in rats with post-traumatic stress disorder and sleep disorder
10.13699/j.cnki.1001-6821.2018.10.019
- VernacularTitle:四逆散对创伤后应激障碍及睡眠障碍大鼠海马区神经元放电波幅的影响研究
- Author:
Yue-Feng LI
1
;
Xin-Lei SI
;
Rui CAO
;
Jiang-Tao NIU
;
Ze-Guo ZHANG
;
Tian-Tian BIAN
;
Xing-Ke YAN
Author Information
1. 甘肃中医药大学药学院
- Keywords:
Sinisan;
post-traumatic stress disorder;
hippocampus;
discharge amplitude
- From:
The Chinese Journal of Clinical Pharmacology
2018;34(10):1203-1205
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the function mechanism of the Sinisan on hippocampal neuron discharge amplitude in rats with post-traumatic stress disorder and sleep disorder.Methods Fifty SD rats were divided into blank group,model group,negative control group,positive control group and experimental group.The blank group did not copy the model,did not receive treatment,the normal feeding.The repetitive post-traumatic stress disorder model was induced by current stimulation in model group,but not treated.Negative control group was given equal volume of 0.9％ NaC1.Positive control group was given paroxetine hydrochloride 0.42 mg · mL-1 and experimental group was given Sinisan,containing crude drug 0.24 g · mL-1.The rats were administered with 10 mL · kg-1,once a day,for 7 d.Theneural coding on amplitude in hippocampal CA1/CA3 were detected by M-NEMEA after the treatment.Results The amplitude of the wave amplitude in model group were 63.73 ± 5.80,63.24 ± 6.29,significantly lower than those of negative control group,which were 90.95 ± 5.94,91.16 ± 8.18 (P ＜ 0.01).And the wave amplitude in positive control group were 87.12 ± 8.14,87.21 ± 7.94,and were 91.63 ± 7.30,91.39 ± 7.64 in experimental group,had significant difference with those in model group (P ＜ 0.01).Conclusion The Sinisan have significant positive effect on hippocampal neuron discharge amplitude.
