Effects of Angelica sinensis radix on gastrointestinal hormone and expresomatostatinion of tyrosine kinase receptor C-kit in mouse with blood-deficiency constipation
10.13699/j.cnki.1001-6821.2018.10.017
- VernacularTitle:当归对血虚便秘模型小鼠胃肠激素与酪氨酸激酶受体C-kit表达的影响
- Author:
Li-Dong DU
1
;
Ting-Hui NIU
;
Guo-Tai WU
;
Wu-Zhou LIU
;
Jing SHAO
;
Yuan REN
Author Information
1. 甘肃中医药大学药学院
- Keywords:
Angelica sinensis;
blood-deficiency and constipation;
gastrointestinal hormone;
C-kit expression
- From:
The Chinese Journal of Clinical Pharmacology
2018;34(10):1195-1198
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effects of Angelica sinensis radix (Danggui) on gastrointestinal hormone and expresomatostatinion of tyrosine kinase receptor C-kit in mouse with blood-deficiency constipation (Xuexu Bianmi).Methods Kunming mouse were randomly divided into six groups,each group had 10 mouse:normal group,model group,control group,three dose of experimental groups.Except the normal group,the mouse in rest groups were orally administrated every day with diphenoxylate 25mg · kg-1 and subcutaneously injected at 1,4,7,10 d with acetylphenyhydrazine 60 mg · kg-1 and intraperitoneally injected at 10-14 d with cyclophosphamide 40 mg · kg-1 to copy Xuexu Bianmi model.At the 14 d,normal and model group were administered physiological saline,the control group were administered solution of Changtongshu Keli 5 mg · kg-1,three dose of experimental groups were administered with different doses of Danggui decoction (4.17,8.33,16.67 g · kg-1).The levels of motilin(MLT),substance P(SP),vasoactive intestinal peptide(VIP),cholecystokinin-8 (CCK-8) and somatostatin in blood were measured by enzyme-linked immunosorbent(ELISA).The expression of C-kit in colon were measured by immunohistochemical technique.Results MLT (pg · mL-1) in normal group,model group,control group,three dose of experimental groups were 1830 ± 246,1498 ± 198,1722 ± 237,1697 ± 245,1754 ± 199,1865 ± 259,respectively;the SP (ng · L-1) in the 6 groups were 266 ± 30,222 ± 34,259 ± 32,218 ± 28,252 ± 29,268 ± 30,respectively;the VIP (ng · L-1) in the 6 groups were 435 ± 109,579 ± 107,508 ± 105,417 ± 139,413 ± 126,445 ± 101,respectively;CCK-8 (pmol · L-1) in the 6 groups were 229 ±26,138 ± 12,180 ±22,164 ± 16,159 ±26,210 ±31,respectively;SS (n · L-1) in the 6 groups were 100 ±20,138 ±29,93 ± 16,104 ±23,101 ±22,99 ± 16,respectively.Comparison between model group and normal group,the di-fference had significantly (P ＜ 0.01,P ＜ 0.05).Comparison between control group on the CCK-8,SS and model group,the difference had significantly(all P ＜0.01).Comparison between experimental-L group on the VIP,CCK-8,SS and model group,the difference had significantly (all P ＜ 0.05)Comparison between experimental-M group on the MLT,VIP,SS and model group,the difference had significantly (all P ＜ 0.05).Comparison between experimental-H group on the MLT,SP,VIP,CCK-8,SS and model group,the difference had significantly(P ＜0.05,P ＜ 0.01).Comparison with the model group,the expression of C-kit in colon in three doses experimental groups were increased.Conclusion The mechanism of Danggui in treating mouse woth Xuexu Bianmi may be related to adjusted gastrointestinal hormone in serum and colon and increased the expression of C-kit in colon.
