Effect of Bufalin on aerobic glycolysis in colorectal cancer HCT116 cell
10.13699/j.cnki.1001-6821.2018.10.011
- VernacularTitle:蟾毒灵对人结肠癌HCT116细胞有氧糖酵解的影响
- Author:
Yu-Xia YUAN
1
;
Xue HE
;
Zhe LI
;
Xue-Yao TANG
;
Yi-Xi ZHANG
;
Yan-Yan QIU
;
Ze-Ting YUAN
;
Pei-Hao YIN
Author Information
1. 上海中医药大学附属普陀医院
- Keywords:
Bufalin;
colorectal cancer;
cell line HCT116;
aerobic glycolysis
- From:
The Chinese Journal of Clinical Pharmacology
2018;34(10):1172-1174,1182
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of Bufalin on aerobic glycolysis in human colorectal cancer cell.Methods The colorectal cancer cells were divided into Bufalin groups and control group.Bufalin groups were treated with Bufalin at various concentrations(5,10,20,40nmol · L-1) for 48 h and control group was given the same dose of complete medium.The level of intracellular ATP level and cell lacate production were determined by Kit.The C-myc and energy metabolism regulator were measured by Western blot.Results Compared with the control group(1.00),4 concentrations (5,10,20,40 nmol · L-1) Bufalin were 91.69％,78.00％,68.55％,54.03％ on the inhibition of human colon cancer cell line HCT116 in ATP level rate;the 4 concentrations Bufalin were 89.04％,77.27％,59.66％,47.52％ to inhibit HCT116 cell junction formation of lactic acid in colorectal cancer cell line rate,the difference was statistically significant (P ＜ 0.05,P ＜ 0.01).Compared with the control group (1.00),the 4 concentrations Bufalin with HCT116 cells C-myc protein colon cancer cell line expression ratio were 0.95,0.84,0.73,0.68;lactate dehydrogenase A (LDH-A) expression ratio were 0.95,0.90,0.79,0.60,the difference was statistically significant (P ＜ 0.05,P ＜ 0.01).Conclusion Bufalin can inhibit the energy metabolism of colorectal cancer cell,which may be related with the down-regulation of C-myc expression.
