Comparison of minimal inhibitory concentrations of ciprofloxacin against Pseudomonas aeruginosa induced by Pseudomonas quinolone signal and ciprofloxacin
10.13699/j.cnki.1001-6821.2018.09.030
- VernacularTitle:喹诺酮信号分子和环丙沙星诱导铜绿假单胞菌对环丙沙星最低抑菌浓度影响的比较
- Author:
Yan-Ru WANG
1
;
Zhi-Qi ZHANG
;
Ya-Ting GAO
;
Xiao-Xia LI
;
Jian-Bang KANG
;
Li-Xia QIU
;
Jin-Ju DUAN
Author Information
1. 山西医科大学药学院
- Keywords:
Pseudomonas aeruginosa;
Pseudomonas quinolone signal;
ciprofloxacin;
induction in vitro;
minimal inhibitory concentration
- From:
The Chinese Journal of Clinical Pharmacology
2018;34(9):1115-1118
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate minimal inhibitory concentration (MIC) of ciprofloxacin against Pseudomonas aeruginosa induced by Pseudomonas quinolone signal (PQS) and ciprofloxacin in vitro.Methods Clinical isolates of Pseudomonas aeruginosa sensitive to ciprofloxacin were collected and then induced ciprofloxacin with three concentrations of 0.5 × MIC,2 × MIC and 4 × MIC,and PQS with three concentrations of 10,40,80 μmol· L-1,respectively for five days.The agar dilution method was used to measure MICs of all strains before and after inductions to ciprofloxacin.The MICs to ciprofloxacin before and after inductions of the same induction scheme were analysed by repeated measures analysis of variance and Paired t-test was used to compare the MICs of two induced schemes.Results Twelve clinical isolates of Pseudomonas aeruginosa sensitive to ciprofloxacin were obtained,among them one was used as the quality control strain included.There had interaction between induction time and induction concentrations of PQS or ciprofloxacin (P ＜0.001 or P ＜0.05).MICs of strains to ciprofloxacin of two induced schemes had statistically significant difference (P ＜ 0.05).Conclusion Under different concentrations of PQS,the trend of MIC values of ciprofloxacin to Pseudomonas aeruginosa varied by induction time.Under different concentrations of ciprofloxacin,MIC values tended to increase with the prolongation of induction time.The effects of two induction schemes on MIC of ciprofloxacin were different.
