Effects of microRNA-451 expression on proliferation ability and apoptosis of ovarian cancer cells
10.13699/j.cnki.1001-6821.2018.09.025
- VernacularTitle:微小RNA-451表达对卵巢癌细胞增殖能力及凋亡水平的影响
- Author:
Xiao-Feng LV
1
;
Han-Qing HONG
;
Shi-Hong CUI
;
Chen-Chen REN
Author Information
1. 郑州大学第三附属医院妇二科
- Keywords:
microRNA-451;
ovarian cancer;
proliferation;
activating transcription factor-2
- From:
The Chinese Journal of Clinical Pharmacology
2018;34(9):1095-1099
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the expression of microRNA-451 (miR-451) in human ovarian cancer tissue and its effect on ovarian cancer cell proliferation and apoptosis.Methods The miR-451 and miR-NC cells of logarithmic growth were taken respectively,and the cell suspension was made by cold PBS solution,and the final concentration was 2.0 x 107 cell · mL-1.The 0.2 cell · mL-1 were inoculated into the subcutaneous tissue of the lower right lower limb of the nude mouse,and the model of subcutaneous tumor was established.Six 5-week old BLAB/c nude mice were randomly divided into two groups:the miR-451 group and the control group,and each group had three nude mouse.In the mir-451 group,miR-451 slow virus supernatant 1 mL,complete culture medium 2 mL and ploybrene 15 g were added to each hole.In the control group,miR-NC slow virus supematant 1 mL,complete culture medium 2 mL and ploybrene 15 g were added to each pore.After 5 weeks of obtaining animal subcutaneous tumor,the expression level of miR-451 in ovarian cancer tissues was detected by Polymerase Chain Reaction.The apoptosis rate of SKVO3 cells was detected by flow cytometer.The gross tumor volume of nude mouse was detected through construct the nude mice subcutaneous tumor model.Changes of ATF2 expression in SKVO3 cells After overexpression of miR-451 was detected by RqPCR and Westen bloting.Results The expression level of miR-451 with ovarian cancer (0.39 ± 0.03) was significantly lower than that in the adjacent tissues of low cancer (1.25 ± 0.03),and the difference was statistically significant (P ＜ 0.05).After expressing miR-451 expression,the apoptosis rate of SKVO3 cells in ovarian cancer (57.10 ± 8.92) ％.Compared with the control group (21.08 ± 5.04),the difference was statistically significant (P ＜ 0.01).The total tumor volume of miR-451 group (1.35 ± 0.23) cm3 was significantly lower than that in the mir-nc group (0.40 ±0.08) cm3,the difference was statistically significant (P ＜0.01).In the tumor tissue of overexpression level of miR-451,immunohistochemical staining intensity of activating transcription factor 2 (ATF2) was decreased significantly(P ＜ 0.05).Conclusion The expression of miR-451 in ovarian cell carcinoma was decreased,and the upregulation of miR-451 could inhibit the expression of ATF2,thus playing a role in anti-tumor growth.
