Effects of Sinisan on sleep latency in rats with post traumatic stress and sleep disorder
10.13699/j.cnki.1001-6821.2018.09.023
- VernacularTitle:四逆散对创伤后应激障碍及睡眠障碍大鼠睡眠潜伏期的影响
- Author:
Yue-Feng LI
1
;
Xin-Lei SI
;
Tian-Tian BIAN
;
Jiang-Tao NIU
;
Rui CAO
;
Xing-Ke YAN
Author Information
1. 甘肃中医药大学药学院
- Keywords:
Sinisan;
post-traumatic stress disorder;
sleep latency;
sleep disorder
- From:
The Chinese Journal of Clinical Pharmacology
2018;34(9):1089-1091
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effects of sleep latency to the intervention of Sinisan in rats with post-traumatic stress and sleep disorder (PTSD).Methods Fifty SD rats were divided equally into 5 groups:sham operation group,model group,negative control group group,positive control group and experimental group.PTSD model was made by claustrophobia,but not in sham operation group.The model group was not given the drug,the negative control group was given equal volume 0.9％ NaC1,and the positive control group was given paroxetine hydrochloride 0.42 mg · mL-1.The experimental group was perfused with the decoction of Sinisan (containing 0.24 g · mL-1) 10 mL · kg-1.The drug was administered 1 h before the stress model was administered once a day for a total of 7 d.After intervention on the 7th day,nonrapid eye movements sleep (NREMS) and eye movements sleep (REMS) were detected.Results The REMS and NREMS of sham operation group and model group were respectively (8.66 ± 3.04),(23.27 t 10.15) min;(65.90 ± 25.08),(109.36 ± 43.43) min,the differences between groups were statistically significant(all P ＜ 0.01);the result suggest that difficulty in falling asleep appears in rats after modeling.The REMS and NREMS of the positive control group and the experimental group were respectively (8.17 ±2.29),(6.83 ±2.84) min;(162.29 ±46.19),(195.24 ±67.96) min.Comparison between the drugs groups and model group,the difference was statistically significant (P ＜0.05,P ＜0.01).These Results suggested that both paroxetine hydrochloride and Sinisan can significantly promote the sleep of rats.Conclusion Traditional Chinese medicine compound Sinisan can obviously promote the rats with sleep disorder caused by PTSD.
