Protective effect of astragaloside Ⅳ on the oxidative stress injury of human glomerular mesangial cells under high glucose conditions and its mechanism
10.13699/j.cnki.1001-6821.2018.09.022
- VernacularTitle:黄芪甲苷对高糖环境下人肾小球系膜细胞的氧化应激损伤的保护作用及机制
- Author:
Lin-Lin WANG
1
;
Jia-Li FENG
;
Jun-Fen LIU
;
Xiu-Qing HAO
;
Lu-Guang ZUO
;
Sheng-Jun LIU
;
Hua LIU
Author Information
1. 河北北方学院附属第一医院肾内科
- Keywords:
glomerular mesangial cell;
high glucose conditions;
astragaloside Ⅳ;
oxidative stress;
mechanism
- From:
The Chinese Journal of Clinical Pharmacology
2018;34(9):1085-1088
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the protective effect of astragaloside Ⅳ on the oxidative stress injury of human glomerular mesangial cells under high glucose conditions and the mechanism.Methods The glomerular mesangial cells were dividedinto model group,control group and experimental-L/M/H groups.Model group was treated with 25 mmol · L-1 glucose,experimental-L/M/H groups were given 25,50,100 μmol · L-1 astragaloside Ⅳ on the basis of model group,control group was treated with equivalent normal saline.Each group was cultured for 48 h after inervention.The cells proliferation were detected by methyl thiazolyl tetrazolium (MTF),the reactive oxygen species (ROS) level and fluorescence intensity were detected by fluorescent probe method,the cells Akt/NF-κB signaling pathways related proteins were detected by Western blot.Results The proliferation inhibition rates of glomerular mesangial cells in experimental-L/M/H groups were (6.79 ± 0.12) ％,(17.05 ± 0.15) ％,(28.31 ± 0.23) ％,and increased with the increasing of astragaloside Ⅳ concentration(P ＜0.01).The ROS relative expressions of glomerular mesangial cells in control group,model group and experimental-L/M/H groups were 0.02 ± 0.01,0.07 ± 0.01,0.06 ± 0.02,0.04 ± 0.03,0.02 ± 0.02;the phospho-protein binases (p-Akt) protein relative expression were 0.25 ± 0.03,0.57 ± 0.02,0.46 ± 0.02,0.31 ± 0.04,0.27 ± 0.03;p-IκBαprotein relative expression were 0.44 ± 0.03,0.16 ±0.01,0.21 ±0.02,0.26 ±0.02,0.33 ±0.02.Compared with control group,the ROS relative expression,fluorescence intensity and p-Akt protein relative expression of model group increased significandy (P ＜ 0.01),the p-IκBα protein relative expression decreased significantly (P ＜0.01).Compared with model group,the ROS relative expression,fluorescence intensity and p-Akt protein relative expression of experimental groups decreased significantly with the increasing of astragaloside Ⅳ concentration (P ＜ 0.01);while the p-IκBα relative expression increased with the increasing of astragaloside Ⅳ concentration (P ＜ 0.01).Conclusion Astragaloside Ⅳ can adjust the ROS expression level of glomerular mesangial cells under high glucose conditions effectively,and inhibit the oxidative stress reaction,the action mechanism maybe related to the down-regulation of p-Akt protein and the up-regulation of p-IκBα protein of Akt/NF-κB signaling pathways,which has significant dose-dependent manner.
