Function of phosphorylated signal transducer and activator of transcription 3 in alcoholic liver injury of mice
10.13699/j.cnki.1001-6821.2018.09.018
- VernacularTitle:磷酸化的信号转导及转录激活因子3在小鼠乙醇性肝损伤过程中的作用
- Author:
Miao-Shui BAI
1
;
San-Qiang LI
;
Ying SONG
;
Xiao-Ping LI
;
Shan-Long WANG
;
Yong-Yong ZHANG
Author Information
1. 河南科技大学医学院肝损伤与修复分子医学重点实验室
- Keywords:
alcoholic liver injury;
phosphorylated signal transducer and activator of transcription 3;
acute phase response;
regenerate
- From:
The Chinese Journal of Clinical Pharmacology
2018;34(9):1070-1072
- CountryChina
- Language:Chinese
-
Abstract:
Objective To research the role of phosphorylated signal transducer and activator of transcription 3 (p-STAT3) in the pathogenesis of alcoholic liver injury in mice.Methods Healthy and mature male mice were reared normally.They were divided at random into 5 groups,namely the normal group (n =10),the experimental-1 group (n =10),the experimental-2 group (n =10),the experimental-3 group(n =10),the experimental-4 group(n =10).The normal group was not treated.The mouse in experimental-1 group,experimental-2 group,experimental-3 group,experimental-4 group were given uninterruptedly 56 degree Beijing Red Star Erguotou by intragastric administration(14 mL · kg-1) for preparation of alcohol liver injury in mice.At the first week,the second week,the third week and the fourth week,respectively,the blood of mice was taken directly by eyeball enucleation.The serum levels of aspartate aminotransferase(AST) was measured with a aspartate aminotransferase assay kit.The expression levels of p-STAT3 in liver of mice was measured by Western blot.Results The serum levels of AST in normal group,experimental-1 group,experimental-2 group,experimental-3 group,experimental-4 group were (112 ±22),(126 ±24),(967 ±30),(1010 ±35),(206 ±23) U · L-1;comparison between experimental-1 group,experimental-4 group and normal group,the difference were without significantly (all P ＞ 0.05);comparison between experimemal-2 group,experimental-3 group and normal group,the difference had significantly (all P ＜ 0.01).The expression levels of p-STAT3 in the 5 groups were (0.52 ± 0.11),(3.21 ±0.33),(1.12 ± 0.21),(1.51 ± 0.23),(2.13 ± 0.26) AU;comparison between experimental-1 group,experimental-4 group and normal group,the difference had significantly (all P ＜ 0.01);comparison between experi mental-2 group,experimental-3 group and normal group,the difference had significantly(all P ＜0.05).ConclusionP-STAT3 may be involved in the acute phase response in the early stage of liver injury in mice,and participate in and promote hepatocyte injury.In the later stage,it may be beneficial to the regeneration,repair and inhibition of hepatocyte apoptosis.
