Influence of different Mueller-Hinton agar on the susceptibility test methods in vitro to tigecycline of Acinetobacter baumannii
10.13699/j.cnki.1001-6821.2018.09.015
- VernacularTitle:不同Mueller-Hinton培养基对鲍曼不动杆菌替加环素体外药敏结果的影响
- Author:
Yuan-Hui HE
1
;
Ling-Yun ZHANG
;
Yun LI
;
Sai-Nan ZHU
;
Feng XUE
;
Bo ZHENG
Author Information
1. 北京大学第一医院临床药理研究所
- Keywords:
Acinetobacter baumannii;
tigecycline;
in vitro susceptibility;
Muller-Hinton medium
- From:
The Chinese Journal of Clinical Pharmacology
2018;34(9):1060-1063
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the Muller-Hinton (MH) mediums from different manufactures and the differences in the activity of tigecycline against Acinetobacter baumannii by different susceptibility test methods in vitro.Methods Testing the activity of tigecycline against 160 strains of clinical isolated Acinetobacter baumannii in vitro by microdilution method when choosing Oxoid MH Broth medium and by agar dilution method or disk diffusion method when choosing Oxoid MH Agar (Oxoid MHA),Oxoid MH Broth + 1.5％ Oxoid Agar(Oxoid MHB + Agar),Becton Dickinson BBL MH Ⅱ Agar(BD MHA) and bioMérieux MH Ⅱ Agar(bioMérieux MHA),respectively.Using atomic absorption spectroscopy method to determine the concentration of calcium,copper,lead,magnesium,cadmium,manganese,iron,nickel and zinc in different medium,simultaneously.Results The susceptibility of tigecycline to Acinetobacter baumannii are 34.4％/53.8％,100.0％/96.9％,63.8％/62.5％,35.6％/29.4％ by agar dilution method or disk diffusion method when using Oxoid MHA,Oxoid MHB + Agar,BD MHA and bioMérieux MHA,respectively,while the susceptibility of tigecycline to Acinetobacter baumannii is 91.3％ by microdilution method when choosing Oxoid MHBroth medium.Conclusion Compared with the results of other MHA mediums,using Oxoid MHB + 1.5％ Agar medium by disk diffusion method can provide a more similar result to those of the broth dilution method when test the activity of tigecycline against Acinetobacter baumannii in vitro.
