Effects of gecko active components on proliferation and reactive oxygen species in HepG2 cells
10.13699/j.cnki.1001-6821.2018.08.014
- VernacularTitle:壁虎活性组分对HepG2细胞增殖及活性氧的影响
- Author:
Yi-Meng DUAN
1
;
Leng-Xin DUAN
;
Ling LIU
;
Meng-Li GUO
;
Bing-Bing WANG
;
Ze-Yue HUANG
;
Jian-Gang WANG
Author Information
1. 河南科技大学 医学院 药理学与分子生物学实验室
- Keywords:
Gecko active components;
reactive oxygen species;
calcium;
mitochondrial membrane potential
- From:
The Chinese Journal of Clinical Pharmacology
2018;34(8):964-967
- CountryChina
- Language:Chinese
-
Abstract:
Objective The aim of this study was to investigate the effects of gecko active components (GACs) on the levels of reactive oxygen species (ROS),calcium and mitochondrial membrane potential (MTP) in HepG2 cells.Methods The experiment was divided into 4 groups:control group (GACs,0),low,middle and high dose group (0.1,0.2,0.3 mg · mL-1,GACs).The growth inhibitory effect of GACs on HepG2 cells were assessed by 3-(4,5-dimethylthiazol-2yl)-2,5-diphenyltetrazolium bromide (MTT) assay.Intracellular reactive oxygen species (ROS) level,calcium level,mitochondrial membrane potential (MMP) level was measured by flow cytometry.Results The results showed that GACs could inhibit the proliferation of HepG2 cells in a dose-dependent manners.After treatment of GACs for 24 h,the 50％ inhibitory dose (IC50) values were 0.21 mg · mL-1.The cell growth inhibition rates of the control group,low-,middle-and high-dose groups were (0.07 ±0.01)％,(0.17 ±0.03)％,(0.49 ±0.12)％ and (0.67 ± 0.18) ％,respectively;the ROS levels of control group,low-,middle-and high-dose groups were (16.61 ± 1.12),(85.81 ±2.56),(268.91 ±2.34),(1741.5 ±5.64);the calcium levels of control group,low,middle and high-dose groups were (9.67 ±O.98),(12.30 ± 1.07),(94.80 ± 2.75),(910.99 ± 5.31);the MMP levels of control group,low-,middle-and high-dose groups were (27.02±1.8) ×10-2,(23.78 ±1.2) ×10-2,(18.27 ±1.5) ×10-2,(16.49 ±2.1) × 10-2,and significant differences were found in the above indexes between low/middle/high dose group and the control group(P ＜0.05 or P ＜0.01).Conclusion GACs can inhibit the proliferation of HepG2 cells,which may cause the increase of ROS content and calcium level and the decrease of MMP,and disrupt the normal function of mitochondria,eventually leading to the apoptosis of HepG2 cells.
