Protective effect of prostaglandin E1 on renal injury in rats with obstructive jaundice
10.13699/j.cnki.1001-6821.2018.08.013
- VernacularTitle:前列腺素E1对梗阻性黄疸大鼠肾损伤的保护作用
- Author:
Jun LIU
1
;
Jin-Fang ZHENG
;
Zhuo-Ri LI
;
Cheng YU
Author Information
1. 海南省人民医院 肝胆胰外科
- Keywords:
obstructive jaundice;
prostaglandin E1;
imflammatory factor;
nuclear factor-κB;
apoptosis index
- From:
The Chinese Journal of Clinical Pharmacology
2018;34(8):961-963,967
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the protective effect of prostaglandin E1 on renal injury in rats with obstructive jaundice.Methods The Wistar rats were randomly divided into three groups (n =15):sham operation group,model group and experimental group.The rat model of obstructive jaundice was established by double ligation of common bile duct.The experimental group rats were injected intraperitoneally with prostaglandin E1 0.1 μg · kg-1 on the seventh postoperative day and treated for 14 d.The other two groups were given the same dose of normal saline.The expression of nuclear factor-κB (NF-κB),B lymphocyto tumor-2 (Bcl-2) and intercellular adhesion molecule-1 (ICAM-1) protein were detected by immunohistochemistry.The tubular epithelial apoptosis index was detected by TdT-mediated dUTP nick-end labeling method.Results The expression of NF-κB protein in sham operation group,model group and experimental group were 1.16 ±0.24,9.28 ± 0.48 and 6.52 ± 0.72;the expression of Bcl-2 protein in the three groups were 0.86 ± 0.54,11.28 ± 0.27 and 8.36 ± 0.48;the expression of ICAM-1 protein in the three groups were 1.04 ± 0.52,18.80 ± 3.26 and 11.18 ± 4.62;the renal tubular epithelial cell apoptosis index in the three groups were O,(5.58 ± 0.46)％ and (1.28 ± 0.24) ％.Comparison between sham operation group and model group,the expression of NF-κB,Bcl-2,ICAM-1 protein and cell apoptosis index was significant (all P ＜ 0.05);comparison between model group and experimental group,the diffrence of the factors was significant (all P ＜ 0.05).Conclusion Prostaglandin E1 had a protective effect on renal injury in rats with obstructive jaundice.The mechanism of the protective effect is related to inhibit the activation of NF-κB and decrease the expression of inflammatory factors.
