Inhibition effects of Yiai capsules on tumor of xenografts in nude mice transplanied hunman lung cancer cell NCI-H460
10.13699/j.cnki.1001-6821.2018.08.012
- VernacularTitle:依癌胶囊对人肺癌NCI-H460细胞裸鼠移植瘤的抑制作用
- Author:
Yun-Ni WEI
1
;
Liu-Ping WANG
;
Yao-Ling LIAO
;
Bin YANG
Author Information
1. 广西医科大学 药学院 药理学教研室
- Keywords:
Yiai capsule;
NCI-H460 cell;
xenografts in nude mice;
inhibitory effect;
cell apoptosis
- From:
The Chinese Journal of Clinical Pharmacology
2018;34(8):957-960
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of Yiai capsules on human lung cancer NCI-H460 cells of xenografts in nude mice.Methods Subcutaneous transplantation tumor model of xenografts in nude mice was established.The nude mouse model were randomly into 5 groups:model group,positive control group,and low,medium,high does experimental groups,8 nude mice in each group.The model group was given equal volume of saline with stomach perfusion.The positive control group was injected intraperitoneally(ip) with cyclophosphamide 20 mg · kg-1,three times a week.The mouse in low,medium,high does experimental groups were received Yiai capsules 0.9,1.8,3.6 g · kg-1,pid,respectively,stomach perfusion.Duration of drugs groups were given drugs for two weeks.At the end of the treatment,the nude mice were sacrificed and the tumor weight of nude mice were weighed and the volume of the transplanted tumor was measured.Morphological changes were observed by hematoxylin eosin (HE) method.The apoptosis rate in each group was detected by TdT-mediated dUTP nick-end labeling (TUNEL) method.Results The inhibitory rates in the model group,positive control group and experimental-L,experimental-M,experimental-H groups were 0,60.30％,18.00％,33.70％,36.30％,respectively.The tumor volume of nude mice in the5 groups were (2879.40 ±546.64),(1124.10 ±256.21),(2048.69 ±747.35),(2017.54 ±719.33),(1642.65 ±546.55)mm3,respectively.Comparison between drugs group and the model group,the difference on the tumor volume was significant (all P ＜ 0.01).The tumor weight in the 5 groups were (2.67 ± 0.59),(1.06 ± 0.31),(2.19 ± 0.88),(1.77 ± 0.82),(1.70 ± 0.89) g,respectively.Comparison between experimental-M,experimental-H groups,positive control group and the model group,the difference on the tumor weight was significant (all P ＜ 0.05).Under the optical microscope,the cells morphology of the model group had no obvious change.The cell morphology of experimental groups and control group changed significantly,and there were different degrees of cell necrosis.After administration,the apoptotic rate of tumor cells in the 5 groups were (5.40 ± 0.02)％,(29.00 ±0.01)％,(11.00 ± 0.03) ％,(16.20 ± 0.01) ％,(22.20 ± 0.02) ％,respectively.Comparison between drugs groups and the model group,the difference on the apoptotic rate had significantly (all P ＜ 0.01).Conclusion The Yiai capsule can inhibit NCI-H460 cells in nude mice transplanted tumor growth.
