Relationship between glutathione peroxidase-1 and cisplatin resistance in human endometrial carcinoma cells and its related mechanism
10.13699/j.cnki.1001-6821.2018.08.011
- VernacularTitle:谷胱甘肽过氧化物酶-1与人子宫内膜癌顺铂抵抗的关系及相关机制研究
- Author:
Lin-Fen XU
1
;
Rong ZHAO
;
Mei-Xian FANG
;
Li-Dong FU
;
Ming-Ying LIN
;
Mei-Shuang LIN
;
Jia-Feng LI
Author Information
1. 福建省妇幼保健院、福建医科大学附属医院妇科
- Keywords:
endometrial carcinoma;
glutathione peroxidase-1;
hypoxia inducible factor-1 α;
chemotherapy resistance
- From:
The Chinese Journal of Clinical Pharmacology
2018;34(8):953-956
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of glutathione peroxidase-1 (GPX1) on cispatin resistance in endometrial carcinoma cells and its related mechanism.Methods The HEC-1-A cisplatin resistance (HEC-1-A CR)cell was constructed by intermittent concentration gradient method.The control HEC-1-A cell was constructed with equal amount of normal saline.Lentivirus transfection was used to up-regulate the expression of GPX1 in the HEC-1-A CR cell as HEC-1-A CR GPX1 cell,and blank vector transfected was regarded as HEC-1-A CR control cell.The half maximal inhibitory concentration (IC50) value of cisplatin in cells was detected by thiazolyl blue (MTS) assay.The expression of mRNA and protein of GPX1 and HIF-1 α were detected by fluorescent quantitative chain reaction (qPCR) and Western blotting.Results The IC50 of HEC-1-A cells and HEC-1-A CR cells were (2.13 ±0.24),(7.89 ± 1.17)μg · mL-1.The IC50of HEC-1-A CR control cells and HEC-1-A CR GPX1 cells were (7.91 ±0.98),(3.87 ±0.52)μg · mL-1.The expression level of GPX1 mRNA in HEC-1-A cells and HEC-1-A CR cells were 0.14 ±0.01,0.07 ±0.01.The expression level of HIF-1α mRNA in HEC-1-A cells and HEC-1-A CR cells were 0.32 ±0.03,0.72 ±0.08.The expression level of GPX1 mRNA in HEC-1-A CR control cells and HEC-1-A CR cells were 0.09 ± 0.02,0.24 ± 0.03.The expression level of HIF-1 α mRNA in HEC-1-A CR control cells and HEC-1-A CR cells were 0.76 ±0.08,0.54 ±0.05.The above indexes were compared between groups,the difference was statistically significant (all P ＜ 0.05).The results of the changes of GPX1 and HIF-1 α protein levels were in accordance with the changes of mRNA levels.Conclusion GPX1 can enhance the sensitivity of cisplatin in endometrial cancer cells via down-regulating the expression of HIF-1 α.
