Improvment effect of metformin on ameliorates hyperglycemia induced by lipopolysaccharide in diet induced obese mice
10.13699/j.cnki.1001-6821.2018.08.009
- VernacularTitle:二甲双胍对牙周致病菌内毒素所致饮食诱导肥胖小鼠高血糖的改善作用
- Author:
Min-Hua WANG
1
;
Shu-Li DENG
;
Yun-Xi LIU
;
Fei-Fei CHEN
;
Hao-Shu WU
Author Information
1. 浙江大学 药学院药理学
- Keywords:
lipopolysaccharides;
metformin;
hyperglycemia;
obesity;
porphyromonas gingivalis
- From:
The Chinese Journal of Clinical Pharmacology
2018;34(8):945-948
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the impact of porphyromonas gingivalis (PG) lipopolysaccharide (LPS) on blood glucose of high-fat (HF) diet-induced obese (DIO) mice and the antagonistic effect of metformin on diabetes model mice.Methods The C57 mice were divided into 8 groups(n =6-8 in each group).Apart from the normal control,mice were fed with HF diet for 16 weeks (including 3 groups) or 21 weeks (including 3 groups) for modeling.Besides the HF control group,the mice of diabetes model group and metformin group were given PG LPS 100 μg · kg-1 with intraperitoneal injection and simultaneously administrated with 100 mg · kg-1 metformin by gavage daily for 3 weeks,respectively.Blood glucose was measured with rapid glucometer.The plasma insulin and tumor necrosis factor-α(TNF-α) were tested by enzyme linked immunosorbent assay.The expression of AMP-activated protein kinase (AMPK) pathway protein in livers were detected by Western blotting.Results In 21 weeks DIO mice,the blood glucose levels in the HF control group,diabetes model group and metformin group were (6.46±0.31),(7.82 ±0.35),(6.23 ± 0.21) mmol · L-1,respectively.Comparison between HF control group and diabetes model group,the difference was significant (P ＜ 0.05);comparison between diabetes model group and metformin group,the difference was significant (P ＜ 0.01).Expression of AMPK-phosphorylation status (p-AMPK)in the diabetes model group and metformin group were 1.03 ± 0.04,1.62 ± 0.21,respectively.Comparison between diabetes model group and metformin group,the difference was significant(P ＜0.05).Conclusion Metformin is a preferred therapeutic agent for hyperglycemic patients with obesity and periodontitis.
