Study on the identification and anti-inflammatory effect of endophyte from Glycyrrhiza uralensis in vitro and in vivo
10.13699/j.cnki.1001-6821.2018.07.035
- VernacularTitle:甘草内生菌的鉴定及抗炎作用研究
- Author:
Zhi-Jun YANG
1
;
Yi DENG
;
Qiong MAN
;
Xiu-Juan YANG
;
Yan-Ze YANG
;
Liang LIU
Author Information
1. 甘肃中医药大学药学院
- Keywords:
Glycyrrhiza uralensis;
endophyte;
identification;
anti-inflammation
- From:
The Chinese Journal of Clinical Pharmacology
2018;34(7):852-856
- CountryChina
- Language:Chinese
-
Abstract:
Objective To identify endophytes from Glycyrrhiza uralensis and study their anti-inflammatory effects in vivo and in vitro.Methods RAW264.7 cells in the logarithm growth period were made 1 × 106 cell · mL-1 to be single cell suspension.The blank group was added with 1640 culture medium,the model group was added with 1 mg mL-1 lipopolysaccharide (LPS)for culture,the control group was added with Glycyrrhiza water extracts(10,20,40 mg · L-1),and experimental group was added with 10,20,40 mg · L-1 endophyte fermentation products (JTYB006,JTYB012,JTYB029,JTYF023) for 2 h of treatment,then 1 nag · L-1 LPS was added for stimulation.The content of nitric oxide (NO) were measured by Griess,and interleukin-1β (IL-1β) and prostaglandin E2 (PGE2) were measured by ELISA.Kunming mice were randomly divided into 7 groups:model,control Ⅰ,control Ⅱ,experimental Ⅰ,experimental Ⅱ,experimental Ⅲ,experimental Ⅳ.Then xylene-induced mouse ear edema test and Glacial acetic acid-induced mouse abdominal capillary permeability increase test were conducted.Results Three strains of endophytic bacterium are Bacillus,belonging to 3 species,and 1 strain of endophytic fungi is Aspergillus ochraceus.The contents of NO,PGE2 and IL-1β were (89.53 ± 8.02) μmol · L-1,(420.85 ± 57.85) pg · mL-1,(35.24 ± 5.77) pg · mL-1 in model group.When the mass concentration was maintained at 40mg· L-1,the contents of NO were (65.60 ±4.31),(63.48 ±6.89),(62.30 ±5.92),(56.47 ±7.26) μmol · L-1,the contents of PGE2 were(254.07 ±43.62),(295.40 ±49.13),(280.65 ±52.91),(296.42 ±54.90) pg·mL-1,the contents of IL-1β were(21.34 ±5.36),(20.65 ±4.78),(16.67 ±3.98),(22.54 ±5.30)pg · mL-1 in 4 experimental groups.Comparison between model group and experimental groups showed that the differences were significant (P ＜0.05 or P ＜0.01).The ear swelling degree and the absorbance of abdominal Flushing fluid in model group were 17.99 ± 1.60,0.28 ±0.02.The ear swelling degree were 15.05 ± 2.38,13.68 ± 2.69,15.36 ±2.03,the absorbance of abdominal Flushing fluid were 0.24 ±0.04,0.18 ±0.05,0.21 ±0.05 in experimental Ⅰ,Ⅲ,Ⅳ groups.By comparison between model group and experimental groups,the differences were significant (P ＜0.05 or P ＜ 0.01).Conclusion The fermentations of endophytes in 3 strains (JTYB006,JTYB029 and JTYF023) have anti-inflammatory effects both in vitro and in vivo.
