Effect of Sinisan on hippocampal neurons signal intensity in rats with post-traumatic stress disorder
10.13699/j.cnki.1001-6821.2018.07.027
- VernacularTitle:四逆散对创伤后应激障碍大鼠海马神经元信号强度的影响
- Author:
Yue-Feng LI
1
;
Tian-Tian BIAN
;
Jiang-Tao NIU
;
Rui CAO
;
Ze-Guo ZHANG
;
Xin-Lei SI
;
Xing-Ke YAN
Author Information
1. 甘肃中医药大学药学院
- Keywords:
Sinisan;
post-traumatic stress disorder;
hippocampus;
power spectral density
- From:
The Chinese Journal of Clinical Pharmacology
2018;34(7):824-826
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the influence of Sinisan on hippocampal CA1 and CA3 neuron spatiotemporal characteristics in rats with post-traumatic stress disorder(PTSD).Methods SD rats were divided into five groups:normal group,blank group,model group,control group,experimental group.Except for normal group and blank group,the rats in other groups were stimulated by the current stimulation plus dark claustrophobic method to replicate the posttraumatic stress disorder (PTSD) model.The blank group and model group were given 0.9％ NaCl 10 mL · kg-1 at 1 h before making the model.The control group and experimental group was given paroxetine hydrochloride 0.42 mg · mL-1 and Sinisan(containing crude drug 0.24 g · mL-1) by intragastric administration,respectively.The model replicated 7 d,and each electric shock was 30 min every morning and afternoon.The neural coding on amplitude in hippocampal CA1 and CA3 were detected by the M-NEMEA after administration,and the spectral density spectrum was obtained from Neuro explorer neural data analysis software.Results The power spectral densities of CA1 and CA3 in hippocampus of rats were determined,they were (-109.08 ± 15.41) and (-108.46 ± 13.16)dB in normal group,(-110.17 ± 14.30)and (-109.54 ± 14.16)dB in blank group,and the differences were not statistically significant (all P ＞ 0.05).Compared with the blank group,the power spectral densities of hippocampus in the model group decreased significantly,which were (-117.39 ± 14.97),(-122.51 ± 16.42) dB,and the differences were statistically significant (all P ＜0.01).Compared with the model group,the power spectral densities of the experimental group in hippocampus were significantly higher (P＜0.01),which were (-110.83 ±11.40),(-110.36 ±10.87)dB.Conclusion The Sinisanhas significant positive effect on hippocampal neuron signal intensity.
