Effects of tetramethylpyrazine combined with cisplatin on transplanted tumor and angiogenesis of Lewis lung adenocarcinoma in mice
10.13699/j.cnki.1001-6821.2018.06.023
- VernacularTitle:川芎嗪联合顺铂对Lewis小鼠肺腺癌移植瘤与血管生成的影响
- Author:
Chang-Hong ZHANG
1
;
Chen LI
;
Bu WANG
;
Li XIAO
;
Su-Jing MA
;
Zhi-Hua ZHANG
Author Information
1. 河北北方学院附属第一医院呼吸内科
- Keywords:
tetramethylpyrazine;
cisplatin;
lung cancer;
vascular endothelial growth factor;
Vasohibin-1
- From:
The Chinese Journal of Clinical Pharmacology
2018;34(6):690-692
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the mechanism of tetramethylpyrazine combined with cisplatin on the transplanted tumor growth and angiogenesis inhibition of Lewis lung adenocarcinoma in mice.Methods Lewis lung carcinoma model of C57BL/6 mice were produced by under arm injection of Lewis lung cancer cells.Then the tumor-bearing mice were randomized into fore groups,with 15 mouse in each group:model group,cisplatin group,tetramethylpyrazine (TMP) group and combined group(TMP + cisplatin).Control group was given with 0.9％ NaCL 0.2 mL,cisplatin group was given with 2 mg· kg-1 cisplatin 0.2 mL,TMP group was given with 100 mg · kg-1 TMP 0.2 mL,combination group was TMP + cisptatin groups,per 0.2 mL.All mouse were sacrificed after intraperitoneal injection for two weeks.Subcutaneous tumors were processed for tumor weight,and the tumor inhibitory rate was calculated.The expressions of vascular endothelial growth factor (VEGF)and Vasohibin-1 (VASH-1) were determined by immunohistochemistry.Results Compared with model group (tumor inhibition rate:0),tumor inhibition rate in combination group,cisplatin group,TMP group were 54.81％,32.36％,18.36％ with statistically significant difference between groups (all P ＜ 0.05).The expression levels of VEGF in combination group,cisplatin group,TMP group,model group were 20.37 ± 2.02,28.07 ± 4.29,26.43 ± 4.69,62.14 ±6.78,respectively.The expression levels of VASH-1 in the four groups were 9.04 ± 1.01,10.10± 1.61,11.53 ± 1.96,12.94 ± 1.10,with statistically significant difference between groups (all P ＜ 0.05).Pearson correlation analysis:the VEGF and VASH-1 was positively correlated (r =0.664,P ＜ 0.05).Conclusion Tetramethylpyrazine has synergetic inhibitory effect with cisplatin on transplanted tumor growth of Lewis lung carcinoma in mice.The action mechanism may be due to exert direct effects on cancer cell and down-regulate the VEGF and VASH-1 expression,inhibiting tumor angiogenesis.
