Study on plasma distribution of microRNA in Chinese Han population
10.13699/j.cnki.1001-6821.2018.06.016
- VernacularTitle:微小RNA在中国汉族人血浆中的分布研究
- Author:
Gao-Feng JIN
1
;
Xi LI
;
Dan-Dan ZHU
;
Wei DING
;
Hong ZHANG
;
Chun-Hua XIA
;
Xin-Hua LI
;
Yu-Qing XIONG
Author Information
1. 南昌大学临床药理研究所
- Keywords:
miRNA-192;
miRNA-206;
miRNA-613;
distribution
- From:
The Chinese Journal of Clinical Pharmacology
2018;34(6):663-666
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a method for extracting and detecting circulating microRNAs(miRNAs) in plasma and to detect the distribution of miR-192,miR-206 and miR-613 in Chinese Han people.Methods The total RNA was extracted from 116 healthy subjects plasma by Trizol LS and then tested by reverse transcription-quantitative polymerase chain reaction (RT-qPCR) method.The expression level of circulating miR-192,miR-206 and miR-613 was standardized by eel-39.Results The △Ct values of circulating miR-192,miR-206 and miR-613 in 116 healthy subjects plasma were 14.01 ± 4.19,17.63 ±4.42 and 19.77 ±4.68,respectively.The expression of plasma circulating miR-192 was significantly higher than miR-206 and miR-613 (P ＜0.05).After grouped by quarrles,the differences of circulating miR-192,miR-206 and miR-613 among four groups were statistically significant (P ＜ 0.05).Conclusion The expression of circulating miR-192,miR-206 and miR-613 in Chinese Han people was distributed normally and showed significant differences.
