Determination the concentration of teicoplanin in human plasma of patients with severe infenction by liquid chromatography tandem mass spectrometry
10.13699/j.cnki.1001-6821.2018.05.024
- VernacularTitle:液相色谱-质谱法测定替考拉宁在重症感染患者血浆中的浓度
- Author:
He YE
1
;
Ying-Chun XIANG
;
Qi HAN
;
Li LI
Author Information
1. 浙江省浙江医院 药剂科
- Keywords:
teicoplanin;
liquid chromatography tandem mass spectrometry;
plasma drug concentration;
pharmacokinetics
- From:
The Chinese Journal of Clinical Pharmacology
2018;34(5):572-575
- CountryChina
- Language:Chinese
-
Abstract:
Objective To develop a liquid chromatography tandem mass spectrometry(LC-MS/MS) method for determination of s teicoplanin in human plasma.Methods The plasma samples were precipitated by methnaol,then the supernatant were separated on Eclipse Plus C1s column (2.1 mm × 100 mm,3.5 μm) and eluted with methnaol-10 mmol · L-1 ammonium acetate(85:15,v/v,containing 0.1％ formic acid),and the column temperature was 25 ℃.Detection of the analyte was achieved using positive ion electrospray ionization (ESI) in the multiple reaction monitoring (MRM) mode.The MS/MS ion transitions monitored were m/z 1880.1→m/z 1563.0,m/z→748.8 m/z 158.1 for teicoplaninand internal standard,respectively.The specificity,standard curve and lower limitation of quantitation,precision and recovery rate,stability and matrix effect were investigated.Results The linear regression equation was y =1.63 × 10-2x-2.95 × 10-3(r =0.9963).The linear range of teicoplanin was 0.10-20.00 μg · mL-1,with the lower limit of quantitation (LLOQ) of 0.10 μg · mL-1.The accuracy was 93.56％-105.12％.Intra-day and inter-dayrelative standard deviations were both below 15％.The absolute recovery in plasma was 80.32％-88.28％.The matrix effect was 91.78％-108.56％.Conclusion The established method is rapid,sensitive,accurate,specific and reliable,and it is suitable for the determination of teicoplanin in human plasma and pharmacokinetic study.
